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| rdf:type
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| Description
| - A high-yield preparatory procedure for the synthesis of p-nitrophenyl 2-acetamido-2-deoxy-b-dgalacto-hexodialdo-1,5-pyranoside (2) using the galactose oxidase from Dactylium dendroides in a batch reactor was developed. Enzymatic recognition of this aldehyde and the respective uronic acid 3 obtained by NaClO2 oxidation was studied using a set of 36 fungal b-N-acetylhexosaminidases from Acremonium, Aspergillus, Penicillium and Talaromyces genera. The aldehyde 2 was readily hydrolysed by all tested b-Nacetylhexosaminidases but neither the uronic acid 3 nor its methyl ester 4 were accepted. Molecular modelling with docking into the active centre of the b-Nacetylhexosaminidase from Aspergillus oryzae revealed that the aldehyde 2 is processed as a C-6 geminal diol by the enzyme
- A high-yield preparatory procedure for the synthesis of p-nitrophenyl 2-acetamido-2-deoxy-b-dgalacto-hexodialdo-1,5-pyranoside (2) using the galactose oxidase from Dactylium dendroides in a batch reactor was developed. Enzymatic recognition of this aldehyde and the respective uronic acid 3 obtained by NaClO2 oxidation was studied using a set of 36 fungal b-N-acetylhexosaminidases from Acremonium, Aspergillus, Penicillium and Talaromyces genera. The aldehyde 2 was readily hydrolysed by all tested b-Nacetylhexosaminidases but neither the uronic acid 3 nor its methyl ester 4 were accepted. Molecular modelling with docking into the active centre of the b-Nacetylhexosaminidase from Aspergillus oryzae revealed that the aldehyde 2 is processed as a C-6 geminal diol by the enzyme (en)
- Byla vyvinuta vysoce účinná metoda pro syntézu 2-acetamido-2-deoxy-b-D-galakto-hexodialdo-1,5-pyranosidu (látka 2) s použitím galaktosaoxidasy z Dactylium dendroides a vsádkového reaktoru. Enzymatické rozpoznání tohoto aldehydu a též odpovídající kyseliny uronové (látka 3) získané oxidací NaClO2 bylo studováno na souboru 36 plísňových beta-N-acetylhexosaminidas z druhů Acremonium, Penicillium a Talaromyces. Aldehyd 2 byl snadno hydrolyzován všemi testovanými enzymy, ale ani kyselina uronová 3 ani její methyl ester nebyly akceptovány. Molekulární modelování do struktury aktivního centra hexosaminidasy z Aspergillus oryzae odhalilo, že aldehyd 2 je pravděpodobně enzymem zpracováván ve formě C-6 geminálního diolu (cs)
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| Title
| - Combined Application of Galactose Oxidase and Beta-N-Acetylhexosaminidase in the Synthesis of Complex Immunoactive N-Acetyl-D-galactosaminides
- Combined Application of Galactose Oxidase and Beta-N-Acetylhexosaminidase in the Synthesis of Complex Immunoactive N-Acetyl-D-galactosaminides (en)
- Použití galaktosaoxidasy v kombinaci s beta-N-acetylhexosaminidasou pro syntézu komplexních imunoaktivních N-acetyl-D-galaktosaminidů (cs)
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| skos:prefLabel
| - Combined Application of Galactose Oxidase and Beta-N-Acetylhexosaminidase in the Synthesis of Complex Immunoactive N-Acetyl-D-galactosaminides
- Combined Application of Galactose Oxidase and Beta-N-Acetylhexosaminidase in the Synthesis of Complex Immunoactive N-Acetyl-D-galactosaminides (en)
- Použití galaktosaoxidasy v kombinaci s beta-N-acetylhexosaminidasou pro syntézu komplexních imunoaktivních N-acetyl-D-galaktosaminidů (cs)
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| skos:notation
| - RIV/61388971:_____/05:00022301!RIV06-AV0-61388971
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA203/04/1045), Z(AV0Z50200510), Z(MSM6007665808)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/05:00022301
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - galactose oxidase; glycosylation (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Advanced Synthesis & Catalysis
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Bezouška, Karel
- Elling, L.
- Křen, Vladimír
- Křenek, Karel
- Kuzma, Marek
- Ettrich, R.
- Fialová, Pavla
- Rauvolfová, Jana
- Namdjou, D.-J.
- Přikrylová, Věra
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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