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Description
  • Byl vypracován rychlý a jednoduchý protokol pro elektroporaci Clostridium perfringens plasmidovou DNA. Zavedené modifikace zvyšují účinnost transformace oproti původním hodnotám o jeden řád (cs)
  • A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude
  • A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude (en)
Title
  • Rapid protocol for electroporation of Clostridium perfringens
  • Rychlý protokol pro elektroporaci Clostridium perfringens (cs)
  • Rapid protocol for electroporation of Clostridium perfringens (en)
skos:prefLabel
  • Rapid protocol for electroporation of Clostridium perfringens
  • Rychlý protokol pro elektroporaci Clostridium perfringens (cs)
  • Rapid protocol for electroporation of Clostridium perfringens (en)
skos:notation
  • RIV/61388971:_____/05:00021777!RIV06-AV0-61388971
http://linked.open.../vavai/riv/strany
  • 125;127
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M0520), P(GA310/02/1436), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika
  • -
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 540032
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/05:00021777
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • clostridium perfringens; electroporation; transformation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [3025897F7155]
http://linked.open...i/riv/nazevZdroje
  • Journal of Microbiological Methods
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 62
http://linked.open...iv/tvurceVysledku
  • Branny, Pavel
  • Ruml, T.
  • Vítek, L.
  • Jirásková, Alena
  • Fevery, J.
http://linked.open...n/vavai/riv/zamer
issn
  • 0167-7012
number of pages
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