| Attributes | Values |
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| rdf:type
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| Description
| - All three tryptophan residues in alfa-subunit of mitochondrial processing peptidase (MPP) were subsequently substituted. While substitutions of Trp223 led to misfolded non-functional protein, mutations of Trp147 and/or Trp481 did not affect the enzyme processing activity. Thus, fluorescence properties of the mutants with fewer tryptophans were used for observation of both a-MPP domain translocation and visualization of conformational changes in the interdomain linker evoked by substrate. We found that in the presence of substrate the C-terminal penultimate Trp481 was approaching Trp223, which is localized at the border of N-terminal domain and interdomain linker. Also, excision of the a-MPP C-terminal 30 amino acid residues (DC30) led to a complete loss of protein function
- All three tryptophan residues in alfa-subunit of mitochondrial processing peptidase (MPP) were subsequently substituted. While substitutions of Trp223 led to misfolded non-functional protein, mutations of Trp147 and/or Trp481 did not affect the enzyme processing activity. Thus, fluorescence properties of the mutants with fewer tryptophans were used for observation of both a-MPP domain translocation and visualization of conformational changes in the interdomain linker evoked by substrate. We found that in the presence of substrate the C-terminal penultimate Trp481 was approaching Trp223, which is localized at the border of N-terminal domain and interdomain linker. Also, excision of the a-MPP C-terminal 30 amino acid residues (DC30) led to a complete loss of protein function (en)
- Všechny tři tryptofanové zbytky v alfa podjednotce mitochondriální procesující peptidasy byly postupně nahrazeny a bylo zjištěno, že zachování zbytku Trp223 je pro funkci a správné složení nezbytný. Další dvě substituce neovlivnily vlastnosti enzymu. Pozměněné proteiny s omezeným počtem tryptofanů byly využity pro pozorování konfirmačních změn obou domén podjednotky při interakci se substrátem. V přítomnosti substrátu dochází v významnému přiblížení zbytků Trp481 a Trp223, které jsou součástí různých domén proteinu (cs)
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| Title
| - Substrate evokes translocation of both domains in the mitochondrial processing peptidase alfa-subunit during which the C-terminus acts as a stabilizing element
- Substrát vyvolává translokaci obou domén alfa-podjednotky mitochondriální procesující peptidasy, při níž působí její C-konec jako stabilizační prvek (cs)
- Substrate evokes translocation of both domains in the mitochondrial processing peptidase alfa-subunit during which the C-terminus acts as a stabilizing element (en)
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| skos:prefLabel
| - Substrate evokes translocation of both domains in the mitochondrial processing peptidase alfa-subunit during which the C-terminus acts as a stabilizing element
- Substrát vyvolává translokaci obou domén alfa-podjednotky mitochondriální procesující peptidasy, při níž působí její C-konec jako stabilizační prvek (cs)
- Substrate evokes translocation of both domains in the mitochondrial processing peptidase alfa-subunit during which the C-terminus acts as a stabilizing element (en)
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| skos:notation
| - RIV/61388971:_____/04:00022937!RIV06-AV0-61388971
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA204/01/1001), Z(AV0Z5020903)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/04:00022937
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - metallopeptidase; mitochondria; mitochondrial processing peptidase (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Biochemical and Biophysical Research Communications
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Kubala, Martin
- Amler, Evžen
- Holá, Klára
- Matušková, Anna
- Janata, Jiří
- Parkhomenko, Natalia
- Kutejová, E.
- Gakh, A.
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
| |
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