About: A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: a crystallographic and computational study

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The nuclease domain of colicin E7 metallonuclease (NColE7) contains its active centre at the C-terminus. The mutant Delta N4-NColE7-C* - where the four N-terminal residues including the positively charged K446, R447 and K449 are replaced with eight residues from the GST tag - is catalytically inactive. The crystal structure of this mutant demonstrates that its overall fold is very similar to that of the native NColE7 structure. This implicates the stabilizing effect of the remaining N-terminal sequence on the structure of the C-terminal catalytic site and the essential role of the deleted residues in the mechanism of the catalyzed reaction. Complementary QM/MM calculations on the protein-DNA complexes support the less favourable cleavage by the mutant protein than by NColE7. Furthermore, a water molecule as a possible ligand for the Zn2+-ion is proposed to play a role in the catalytic process. These results suggest that the mechanism of the Zn2+-containing HNH nucleases needs to be further studied and discussed. The nuclease domain of colicin E7 metallonuclease (NColE7) contains its active centre at the C-terminus. The mutant Delta N4-NColE7-C* - where the four N-terminal residues including the positively charged K446, R447 and K449 are replaced with eight residues from the GST tag - is catalytically inactive. The crystal structure of this mutant demonstrates that its overall fold is very similar to that of the native NColE7 structure. This implicates the stabilizing effect of the remaining N-terminal sequence on the structure of the C-terminal catalytic site and the essential role of the deleted residues in the mechanism of the catalyzed reaction. Complementary QM/MM calculations on the protein-DNA complexes support the less favourable cleavage by the mutant protein than by NColE7. Furthermore, a water molecule as a possible ligand for the Zn2+-ion is proposed to play a role in the catalytic process. These results suggest that the mechanism of the Zn2+-containing HNH nucleases needs to be further studied and discussed. (en)
Title	A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: a crystallographic and computational study A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: a crystallographic and computational study (en)
skos:prefLabel	A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: a crystallographic and computational study A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: a crystallographic and computational study (en)
skos:notation	RIV/61388963:_____/14:00437486!RIV15-GA0-61388963
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA14-31419S)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Rulíšek, Lubomír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	902
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/14:00437486
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	colicin E7 metallonuclease; catalytic activity; crystal structure (en)
http://linked.open.../riv/klicoveSlovo	catalytic activity crystal structure colicin E7 metallonuclease
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[753678788BA1]
http://linked.open...i/riv/nazevZdroje	Metallomics
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Počítačový design minimalistických metalopeptidů jako cesta k rozluštění katalytické účinnosti metaloproteinů
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	6
http://linked.open...iv/tvurceVysledku	Rulíšek, Lubomír Tóth, E. Christensen, H. E. M. Czene, A. Gyurcsik, B. Larsen, S. Nagata, K. Németh, E. Otten, H. Poulsen, J. C. N.
http://linked.open...ain/vavai/riv/wos	000344321300011
issn	1756-5901
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1039/c4mt00195h

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