About: The crystal structure of the phosphatidylinositol 4-kinase II alpha

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Phosphoinositides are a class of phospholipids generated by the action of phosphoinositide kinases with key regulatory functions in eukaryotic cells. Here, we present the atomic structure of phosphatidylinositol 4-kinase type II alpha (PI4K II alpha), in complex with ATP solved by X-ray crystallography at 2.8 angstrom resolution. The structure revealed a non-typical kinase fold that could be divided into Nand C-lobes with the ATP binding groove located in between. Surprisingly, a second ATP was found in a lateral hydrophobic pocket of the C-lobe. Molecular simulations and mutagenesis analysis revealed the membrane binding mode and the putative function of the hydrophobic pocket. Taken together, our results suggest a mechanism of PI4K II alpha recruitment, regulation, and function at the membrane. Phosphoinositides are a class of phospholipids generated by the action of phosphoinositide kinases with key regulatory functions in eukaryotic cells. Here, we present the atomic structure of phosphatidylinositol 4-kinase type II alpha (PI4K II alpha), in complex with ATP solved by X-ray crystallography at 2.8 angstrom resolution. The structure revealed a non-typical kinase fold that could be divided into Nand C-lobes with the ATP binding groove located in between. Surprisingly, a second ATP was found in a lateral hydrophobic pocket of the C-lobe. Molecular simulations and mutagenesis analysis revealed the membrane binding mode and the putative function of the hydrophobic pocket. Taken together, our results suggest a mechanism of PI4K II alpha recruitment, regulation, and function at the membrane. (en)
Title	The crystal structure of the phosphatidylinositol 4-kinase II alpha The crystal structure of the phosphatidylinositol 4-kinase II alpha (en)
skos:prefLabel	The crystal structure of the phosphatidylinositol 4-kinase II alpha The crystal structure of the phosphatidylinositol 4-kinase II alpha (en)
skos:notation	RIV/61388963:_____/14:00435136!RIV15-AV0-61388963
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(LO1302)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Bouřa, Evžen Chalupská, Dominika Nencka, Radim Klíma, Martin Dubánková, Anna Bäumlová, Adriana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	9296
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/14:00435136
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	crystal structure; kinase; membrane; Monte Carlo simulations; phosphatidyl inositol (en)
http://linked.open.../riv/klicoveSlovo	Monte Carlo simulations crystal structure membrane kinase phosphatidyl inositol
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[ED4A9CA43ABD]
http://linked.open...i/riv/nazevZdroje	Embo Reports
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	InterBioMed
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Nencka, Radim Bouřa, Evžen Klíma, Martin Balla, T. Rozycki, B. Chalupská, Dominika Bäumlová, Adriana Dubánková, Anna Jovic, M. Kloer, D. P. Wisniewski, E.
http://linked.open...ain/vavai/riv/wos	000342890700012
issn	1469-221X
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.15252/embr.201438841

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