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  • The review presents a comprehensive survey of recent developments and applications of capillary and microchip electroseparation methods (zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography) for analysis, isolation, purification, and physicochemical and biochemical characterization of peptides. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are presented. New developments in particular CE and CEC modes are reported and several types of their applications to peptide analysis are described: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide relevant physicochemical characteristics of peptides are demonstrated.
  • The review presents a comprehensive survey of recent developments and applications of capillary and microchip electroseparation methods (zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography) for analysis, isolation, purification, and physicochemical and biochemical characterization of peptides. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are presented. New developments in particular CE and CEC modes are reported and several types of their applications to peptide analysis are described: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide relevant physicochemical characteristics of peptides are demonstrated. (en)
Title
  • Recent developments in capillary and microchip electroseparations of peptides (2011-2013)
  • Recent developments in capillary and microchip electroseparations of peptides (2011-2013) (en)
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  • Recent developments in capillary and microchip electroseparations of peptides (2011-2013)
  • Recent developments in capillary and microchip electroseparations of peptides (2011-2013) (en)
skos:notation
  • RIV/61388963:_____/14:00429826!RIV15-GA0-61388963
http://linked.open...avai/riv/aktivita
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  • I, P(GA13-17224S), P(GAP206/12/0453)
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  • 1
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  • 41541
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  • RIV/61388963:_____/14:00429826
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  • capillary electrochromatography; capillary electrophoresis; microchip; peptides; proteins (en)
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  • DE - Spolková republika Německo
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  • [9E18B39C5E31]
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  • Electrophoresis
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  • 35
http://linked.open...iv/tvurceVysledku
  • Kašička, Václav
http://linked.open...ain/vavai/riv/wos
  • 000337366500006
issn
  • 0173-0835
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/elps.201300331
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