About: Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions

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About: Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Effect of Zn2+ ions on the conformation of polyA in cacodilic buffer at pH 7 was investigated by differential UV spectroscopy (DUV) and by thermal denaturation. The shapes of the DUV spectra and melting curves suggest a transition of polyA into a more ordered %22metallized%22, possibly double-helical conformation at Zn2+ concentrations above 3 x 10(-5) M. A phase diagram of polyA complexes with Zn2+ was constructed for the temperature range from 20 degrees C to 95 degrees C and Zn2+ concentrations between 10(-5) M and 5 x 10(-4) M. It was found that the transition of a single strand into the %22metallized%22 form is possible only if the length of the disordered single-stranded region becomes larger than a certain critical value, ranging between 98% and 78% as the metal concentration increases from 3 x 10(-5) to 5 x 10(-4) M. Effect of Zn2+ ions on the conformation of polyA in cacodilic buffer at pH 7 was investigated by differential UV spectroscopy (DUV) and by thermal denaturation. The shapes of the DUV spectra and melting curves suggest a transition of polyA into a more ordered %22metallized%22, possibly double-helical conformation at Zn2+ concentrations above 3 x 10(-5) M. A phase diagram of polyA complexes with Zn2+ was constructed for the temperature range from 20 degrees C to 95 degrees C and Zn2+ concentrations between 10(-5) M and 5 x 10(-4) M. It was found that the transition of a single strand into the %22metallized%22 form is possible only if the length of the disordered single-stranded region becomes larger than a certain critical value, ranging between 98% and 78% as the metal concentration increases from 3 x 10(-5) to 5 x 10(-4) M. (en)
Title	Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions (en)
skos:prefLabel	Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions (en)
skos:notation	RIV/61388963:_____/13:00420878!RIV14-GA0-61388963
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP208/10/0559)
http://linked.open...iv/cisloPeriodika	Oct
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Andrushchenko, Valery
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	71843
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/13:00420878
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	metal ions; polyA; metallized form; differential UV spectroscopy; thermal denaturation; phase diagram (en)
http://linked.open.../riv/klicoveSlovo	metallized form polyA metal ions phase diagram differential UV spectroscopy thermal denaturation
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[FA519D9CAF7A]
http://linked.open...i/riv/nazevZdroje	International Journal of Biological Macromolecules
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Theoretical and spectroscopic studies of nucleic acid self-assemblies
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	61
http://linked.open...iv/tvurceVysledku	Andrushchenko, Valery Sorokin, V. A. Usenko, E. L. Valeev, V. A.
http://linked.open...ain/vavai/riv/wos	000326660700065
issn	0141-8130
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ijbiomac.2013.08.010

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