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  • In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for peptide analysis is demonstrated by two strategies typically followed in analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic dipeptide gama-glutamyl-S-ethenyl-cysteine in a complex matrix of legume plant. The second one is a shotgun-like methodology used for proteomic analysis; particularly, for investigation of the differences between a genetically modified variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics.
  • In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for peptide analysis is demonstrated by two strategies typically followed in analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic dipeptide gama-glutamyl-S-ethenyl-cysteine in a complex matrix of legume plant. The second one is a shotgun-like methodology used for proteomic analysis; particularly, for investigation of the differences between a genetically modified variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics. (en)
Title
  • Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies
  • Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies (en)
skos:prefLabel
  • Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies
  • Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies (en)
skos:notation
  • RIV/61388963:_____/13:00398250!RIV14-GA0-61388963
http://linked.open...avai/riv/aktivita
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  • P(GA203/08/1428), Z(AV0Z40550506)
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  • 64229
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  • RIV/61388963:_____/13:00398250
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  • peptides; peptidomics; proteomics; CE-MS (en)
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  • [6C190AAAFD97]
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  • New York
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  • Methods in Molecular Biology, 984
http://linked.open...i/riv/nazevZdroje
  • Capillary Electrophoresis of Biomolecules: Methods and Protocols
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  • Kašička, Václav
  • Cifuentes, A.
  • Simo, C.
http://linked.open...n/vavai/riv/zamer
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/978-1-62703-296-4_11
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  • Humana Press
https://schema.org/isbn
  • 978-1-62703-296-4
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