About: Raman optical activity study on insulin amyloid- and prefibril intermediate

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The amyloid fibril of bovine insulin and its renaturing intermediates were studied by using Raman optical activity (ROA). In the spectrum of the amyloid, the sharp +/- ROA couplet of amide I band characteristic of the beta-sheet-rich proteins was observed, together with a sharp peak at 1271 cm(-1) characteristic of a turn structure. The shoulder ROA peak of the native insulin at similar to 1340 cm(-1), which was assigned to the hydrated alpha-helix, was not observed in the amyloid, suggesting that the hydrated alpha-helix was converted to the parallel beta-sheet structure in the amyloid. Recovery of the amyloid to the native state was also monitored by ROA. The intermediate states showed distinct features from the amyloid or native ones. The intermediates did not show a characteristic ROA peak of the poly(L-proline) II helix at similar to 1318 cm(-1). The hydrated alpha-helix ROA peak was not recovered in the intermediate states. In a process of the amyloid formation, at first the hydrated alpha-helix of the native insulin is converted to a specific partially unfolded structure, and then, it was converted to the parallel beta-sheet structure with many turns. The amyloid fibril of bovine insulin and its renaturing intermediates were studied by using Raman optical activity (ROA). In the spectrum of the amyloid, the sharp +/- ROA couplet of amide I band characteristic of the beta-sheet-rich proteins was observed, together with a sharp peak at 1271 cm(-1) characteristic of a turn structure. The shoulder ROA peak of the native insulin at similar to 1340 cm(-1), which was assigned to the hydrated alpha-helix, was not observed in the amyloid, suggesting that the hydrated alpha-helix was converted to the parallel beta-sheet structure in the amyloid. Recovery of the amyloid to the native state was also monitored by ROA. The intermediate states showed distinct features from the amyloid or native ones. The intermediates did not show a characteristic ROA peak of the poly(L-proline) II helix at similar to 1318 cm(-1). The hydrated alpha-helix ROA peak was not recovered in the intermediate states. In a process of the amyloid formation, at first the hydrated alpha-helix of the native insulin is converted to a specific partially unfolded structure, and then, it was converted to the parallel beta-sheet structure with many turns. (en)
Title	Raman optical activity study on insulin amyloid- and prefibril intermediate Raman optical activity study on insulin amyloid- and prefibril intermediate (en)
skos:prefLabel	Raman optical activity study on insulin amyloid- and prefibril intermediate Raman optical activity study on insulin amyloid- and prefibril intermediate (en)
skos:notation	RIV/61388963:_____/12:00377069!RIV13-AV0-61388963
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Yamamoto, Shigeki
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	164021
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/12:00377069
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	raman optical activity; amyloid; fibril; intermediate; insulin (en)
http://linked.open.../riv/klicoveSlovo	raman optical activity insulin intermediate fibril amyloid
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[7CDC69BC69D6]
http://linked.open...i/riv/nazevZdroje	Chirality
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	24
http://linked.open...iv/tvurceVysledku	Watarai, H. Yamamoto, Shigeki
http://linked.open...ain/vavai/riv/wos	000298873000001
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství
issn	0899-0042
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/chir.21029

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