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| rdf:type
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| Description
| - Regulation of biomineralization processes are mediated by extracellular matrix proteins that often exhibit proline-rich regions. Advanced bioinformatic methods were used to design the structure of artificial peptides (P1, P2, P3) based on proline-rich domains of extracellular proteins. Their effect on osteoblast differentiation and in vitro biomineralization was tested on MC3T3-E1 and human umbilical cord mesenchymal stem cells (hUCMSCs) and compared to the commercially available enamel matrix derivative (EMD). MC3T3-E1 and hUCMSCs treated with the synthetic peptides showed a decreased cytotoxicity after 24-48 h of treatment compared to control. MC373-E1 cells treated with EMD showed lower expression of osteoblast markers genes than cells treated with P2, except for collagen type I. In hUCMSCs, OC gene expression was higher in P2-treated cells compared to those treated with EMD or control. ALP activity was markedly increased in MC3T3-E1 cells incubated with P2 compared to other treatments. Similar results were observed in hUCMSCs. Further, P2 increased calcium deposition rate compared to EMD or control either in MC3T3-E1 or hUCMSCs. The observed effects of proline-rich peptides hold potential for both clinical applications and as a research tool in further investigations of the molecular basis of induced osteogenic cell differentiation.
- Regulation of biomineralization processes are mediated by extracellular matrix proteins that often exhibit proline-rich regions. Advanced bioinformatic methods were used to design the structure of artificial peptides (P1, P2, P3) based on proline-rich domains of extracellular proteins. Their effect on osteoblast differentiation and in vitro biomineralization was tested on MC3T3-E1 and human umbilical cord mesenchymal stem cells (hUCMSCs) and compared to the commercially available enamel matrix derivative (EMD). MC3T3-E1 and hUCMSCs treated with the synthetic peptides showed a decreased cytotoxicity after 24-48 h of treatment compared to control. MC373-E1 cells treated with EMD showed lower expression of osteoblast markers genes than cells treated with P2, except for collagen type I. In hUCMSCs, OC gene expression was higher in P2-treated cells compared to those treated with EMD or control. ALP activity was markedly increased in MC3T3-E1 cells incubated with P2 compared to other treatments. Similar results were observed in hUCMSCs. Further, P2 increased calcium deposition rate compared to EMD or control either in MC3T3-E1 or hUCMSCs. The observed effects of proline-rich peptides hold potential for both clinical applications and as a research tool in further investigations of the molecular basis of induced osteogenic cell differentiation. (en)
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| Title
| - Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells
- Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells (en)
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| skos:prefLabel
| - Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells
- Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells (en)
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| skos:notation
| - RIV/61388963:_____/11:00389610!RIV13-AV0-61388963
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388963:_____/11:00389610
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - proline-rich regions; synthetic peptides; bone formation; mineralization; In Vitro (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Biomaterials and Tissue Engineering
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Lyngstadaas, S. P.
- Vondrášek, Jiří
- Gaya, A.
- Monjo, M.
- Ramis, J. M.
- Rubert, M.
|
| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| |
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