About: Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode

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About: Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Electrochemical detection of DNA damage in vitro has been used to evaluate the antioxidant properties of transgenic tobacco plant extracts containing metallothionein (MT). This method is based on assessment of efficiency with which the studied plant extracts are able to reduce to the amount of damage of standard chicken DNA by free OH radicals prepared by the Fenton reaction. We have tested the MT isolated from rabbit liver as a standard reference solution and compared its activity with extracts from wild type tobacco plants, and from twelve clones of transgenic tobacco plants variously carrying human or yeast MT. The present experimental results clearly demonstrate that insertion of a MT gene into the DNA of tobacco plants significantly improves their antioxidant properties in comparison with the non transgenic plants. The method developed for the purpose of this study has been demonstrated to be suitable for rapid screening of various plants with respect to their potential for phytoremediation of heavy metals. Electrochemical detection of DNA damage in vitro has been used to evaluate the antioxidant properties of transgenic tobacco plant extracts containing metallothionein (MT). This method is based on assessment of efficiency with which the studied plant extracts are able to reduce to the amount of damage of standard chicken DNA by free OH radicals prepared by the Fenton reaction. We have tested the MT isolated from rabbit liver as a standard reference solution and compared its activity with extracts from wild type tobacco plants, and from twelve clones of transgenic tobacco plants variously carrying human or yeast MT. The present experimental results clearly demonstrate that insertion of a MT gene into the DNA of tobacco plants significantly improves their antioxidant properties in comparison with the non transgenic plants. The method developed for the purpose of this study has been demonstrated to be suitable for rapid screening of various plants with respect to their potential for phytoremediation of heavy metals. (en)
Title	Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode (en)
skos:prefLabel	Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode (en)
skos:notation	RIV/61388963:_____/11:00370762!RIV12-AV0-61388963
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GA102/08/1546), P(GA522/07/0692), Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Macek, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	205728
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/11:00370762
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	square wave voltammetry; carbon paste electrode; DNA; metallothionein (en)
http://linked.open.../riv/klicoveSlovo	square wave voltammetry DNA carbon paste electrode metallothionein
http://linked.open...odStatuVydavatele	CS - Státní svaz Srbska a Černé Hory
http://linked.open...ontrolniKodProRIV	[ABA3A04C9976]
http://linked.open...i/riv/nazevZdroje	International Journal of Electrochemical Science
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Study of thiol compounds production at plants - use in phytoremediation technologies Miniaturized intelligent systems and nanostructured elektrodes for chemical, biological, and pharmaceutical applications (NANIMEL)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	6
http://linked.open...iv/tvurceVysledku	Adam, V. Macek, Tomáš Macková, M. Dioplan, V. Havel, L. Hubálek, J. Kižek, R. Prášek, J. Shetivska, V. Zehnálek, J.
http://linked.open...ain/vavai/riv/wos	000292331400042
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství
issn	1452-3981
number of pages	15 (xsd:int)

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