About: B Family DNA Polymerases Asymmetrically Recognize Pyrimidines and Purines

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We utilized a series of modified pyrimidine analogues to determine the mechanism used by human DNA polymerase α and herpes simplex virus I DNA polymerase to polymerize pyrimidine dNTPs. Removing O2 of a pyrimidine dNTP vastly decreased incorporation by these enzymes and also compromised fidelity in the case of C analogues, while removing O2 from the templating base had more modest effects. Removing the Watson-Crick hydrogen bonding groups greatly impaired polymerization. The Watson-Crick hydrogen bonding plays an important role in enhancing correct dNTP polymerization, but are not essential for preventing misincorporation. These studies also indicate that DNA polymerases recognize bases extremely asymmetrically, both in terms of whether they are a purine or pyrimidine and whether they are in the template or are the incoming dNTP. The mechanistic implications of these results regarding how polymerases discriminate between right and wrong dNTPs are discussed. We utilized a series of modified pyrimidine analogues to determine the mechanism used by human DNA polymerase α and herpes simplex virus I DNA polymerase to polymerize pyrimidine dNTPs. Removing O2 of a pyrimidine dNTP vastly decreased incorporation by these enzymes and also compromised fidelity in the case of C analogues, while removing O2 from the templating base had more modest effects. Removing the Watson-Crick hydrogen bonding groups greatly impaired polymerization. The Watson-Crick hydrogen bonding plays an important role in enhancing correct dNTP polymerization, but are not essential for preventing misincorporation. These studies also indicate that DNA polymerases recognize bases extremely asymmetrically, both in terms of whether they are a purine or pyrimidine and whether they are in the template or are the incoming dNTP. The mechanistic implications of these results regarding how polymerases discriminate between right and wrong dNTPs are discussed. (en)
Title	B Family DNA Polymerases Asymmetrically Recognize Pyrimidines and Purines B Family DNA Polymerases Asymmetrically Recognize Pyrimidines and Purines (en)
skos:prefLabel	B Family DNA Polymerases Asymmetrically Recognize Pyrimidines and Purines B Family DNA Polymerases Asymmetrically Recognize Pyrimidines and Purines (en)
skos:notation	RIV/61388963:_____/11:00364221!RIV12-AV0-61388963
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA400550902), P(LC512), Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika	33
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Hocek, Michal Joubert, Nicolas
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	187728
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/11:00364221
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	active-site tightness; genetic alphabet; deoxynucleoside; phosphoramidites (en)
http://linked.open.../riv/klicoveSlovo	active-site tightness deoxynucleoside genetic alphabet phosphoramidites
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F1F268603062]
http://linked.open...i/riv/nazevZdroje	Biochemistry
http://linked.open...in/vavai/riv/obor	CC
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for biomolecules and complex molecular systems Development of modular syntheses of new C-nucleosides for studying DNA polymerase specificity and for the extension of the genetic alphabet
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	50
http://linked.open...iv/tvurceVysledku	Cavanaugh, N. A. Hocek, Michal Joubert, Nicolas Kuchta, R. D. Lund, T. J. Patro, J. N. Urban, M.
http://linked.open...ain/vavai/riv/wos	000294076100018
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství
issn	0006-2960
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/bi2006916

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