About: Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A series of six pyrimidine modified dNTPs: 5-ethynyl- 5-phenyl- and 5-(3-nitrophenyl)-deoxycitidine and -deoxyuridine triphosphates were prepared and incorporated by primer extension using Vent (exo-) polymerase to specific DNA sequences within or next to the recognition sequences of selected restriction endonucleases. Then the cleavage of these pyrimidine-modified DNA sequences by 13 restriction enzymes was studied. While the presence of any modified C within the target sequence completely prevented any restriction cleavage, most enzymes tolerated the presence of 5-ethynylU and two of them even the presence of 5-phenyl- and 5-(3-nitrophenyl)U. Modifications outside the recognition sequence were tolerated apart from phenyl derivatives with PvuII enzyme. 5-EthynylC was used for protection of the recognition sequence from cleavage in presence of the second unmodified copy of the same sequence that was cleaved. A series of six pyrimidine modified dNTPs: 5-ethynyl- 5-phenyl- and 5-(3-nitrophenyl)-deoxycitidine and -deoxyuridine triphosphates were prepared and incorporated by primer extension using Vent (exo-) polymerase to specific DNA sequences within or next to the recognition sequences of selected restriction endonucleases. Then the cleavage of these pyrimidine-modified DNA sequences by 13 restriction enzymes was studied. While the presence of any modified C within the target sequence completely prevented any restriction cleavage, most enzymes tolerated the presence of 5-ethynylU and two of them even the presence of 5-phenyl- and 5-(3-nitrophenyl)U. Modifications outside the recognition sequence were tolerated apart from phenyl derivatives with PvuII enzyme. 5-EthynylC was used for protection of the recognition sequence from cleavage in presence of the second unmodified copy of the same sequence that was cleaved. (en)
Title	Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases (en)
skos:prefLabel	Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases (en)
skos:notation	RIV/61388963:_____/11:00360993!RIV12-AV0-61388963
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/09/0317), P(LC512), Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Macíčková-Cahová, Hana Pohl, Radek Hocek, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	190552
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/11:00360993
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	base-modified DNA; DNA cleavage; DNA polymerases; nucleosides; restriction endonucleases (en)
http://linked.open.../riv/klicoveSlovo	DNA cleavage DNA polymerases base-modified DNA nucleosides restriction endonucleases
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[C39437650D03]
http://linked.open...i/riv/nazevZdroje	Chembiochem
http://linked.open...in/vavai/riv/obor	CC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Construction of novel functional nucleic acids for applications in chemical biology, catalysis and self assembly. Center for biomolecules and complex molecular systems
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Hocek, Michal Macíčková-Cahová, Hana Pohl, Radek
http://linked.open...ain/vavai/riv/wos	000287718700016
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství
issn	1439-4227
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/cbic.201000644

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