About: 2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential

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About: 2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A comparative study of hypomethylating activities of a series of 5-azacytidine nucleosides: 5-azacytidine, 2´-deoxy-5-azacytidine and its alpha-anomer, 5,6-dihydro-5-azacytidine, 2´-deoxy-5,6-dihydro-5-azacytidine and its α-anomer, and of a 2-pyrimidone ribonucleoside (zebularine) was conducted. Methylation-specific PCR was employed to detect the efficiency of individual agents on cyclin-dependent kinase inhibitor 2B and thrombospondin-1 hypermethylated gene loci. Overall changes in DNA methylation level were quantified by direct estimation of 5-methyl-2´-deoxycytidine 5´-monophosphate by HPLC using digested genomic DNA. Flow cytometric analysis of cell cycle progression and apoptotic markers was used to determine cytotoxicity of the compounds. mRNA expression was measured using qRT-PCR. 2´-Deoxy-5,6-dihydro-5-azacytidine was found to be less cytotoxic and more stable than 2´-deoxy-5-azacytidine at the doses that induce comparable DNA hypomethylation and gene reactivation. A comparative study of hypomethylating activities of a series of 5-azacytidine nucleosides: 5-azacytidine, 2´-deoxy-5-azacytidine and its alpha-anomer, 5,6-dihydro-5-azacytidine, 2´-deoxy-5,6-dihydro-5-azacytidine and its α-anomer, and of a 2-pyrimidone ribonucleoside (zebularine) was conducted. Methylation-specific PCR was employed to detect the efficiency of individual agents on cyclin-dependent kinase inhibitor 2B and thrombospondin-1 hypermethylated gene loci. Overall changes in DNA methylation level were quantified by direct estimation of 5-methyl-2´-deoxycytidine 5´-monophosphate by HPLC using digested genomic DNA. Flow cytometric analysis of cell cycle progression and apoptotic markers was used to determine cytotoxicity of the compounds. mRNA expression was measured using qRT-PCR. 2´-Deoxy-5,6-dihydro-5-azacytidine was found to be less cytotoxic and more stable than 2´-deoxy-5-azacytidine at the doses that induce comparable DNA hypomethylation and gene reactivation. (en)
Title	2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential 2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential (en)
skos:prefLabel	2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential 2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential (en)
skos:notation	RIV/61388963:_____/11:00360024!RIV12-AV0-61388963
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0508), Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Votruba, Ivan Mertlíková-Kaiserová, Helena Tloušťová, Eva Otmar, Miroslav Günterová, Jana Matoušová, Marika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	243997
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/11:00360024
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	epigenetic therapy; nucleoside analogs; DNA methylation; 2'-deoxy-5-azacytidine; 2'-deoxy-5,6-dihydro-5-azacytidine (en)
http://linked.open.../riv/klicoveSlovo	nucleoside analogs 2'-deoxy-5 2'-deoxy-5-azacytidine 6-dihydro-5-azacytidine epigenetic therapy DNA methylation
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[1D0FE13F483A]
http://linked.open...i/riv/nazevZdroje	Epigenetics
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	New Antivirals and Antineoplastics
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	6
http://linked.open...iv/tvurceVysledku	Mertlíková-Kaiserová, Helena Votruba, Ivan Tloušťová, Eva Otmar, Miroslav Günterová, Jana Matoušová, Marika
http://linked.open...ain/vavai/riv/wos	000291148100013
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství
issn	1559-2294
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.4161/epi.6.6.16215

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