About: Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two capillary electrophoretic methods were developed and evaluated for measurement of glycated hemoglobin HbA1c. Firstly, a capillary zone electrophoresis analysis is performed with a sodium tetraborate buffer of pH 9.3 as background electrolyte in a neutrally coated capillary. HbA1c is separated from HbA0 due to specific interactions of borate anions with the cis-diol pattern in the saccharide moiety of glycohemoglobin. Secondly, a capillary isoelectric focusing method, which exploits a difference in pI values of HbA0 and HbA1c, is performed with Servalyt pH 6-8 or alternatively with Biolyte pH 6-8 carrier ampholytes spiked with a narrow pH cut of pH 7.2 prepared by preparative fractionation of Servalyt pH 4-9 carrier ampholytes. Two capillary electrophoretic methods were developed and evaluated for measurement of glycated hemoglobin HbA1c. Firstly, a capillary zone electrophoresis analysis is performed with a sodium tetraborate buffer of pH 9.3 as background electrolyte in a neutrally coated capillary. HbA1c is separated from HbA0 due to specific interactions of borate anions with the cis-diol pattern in the saccharide moiety of glycohemoglobin. Secondly, a capillary isoelectric focusing method, which exploits a difference in pI values of HbA0 and HbA1c, is performed with Servalyt pH 6-8 or alternatively with Biolyte pH 6-8 carrier ampholytes spiked with a narrow pH cut of pH 7.2 prepared by preparative fractionation of Servalyt pH 4-9 carrier ampholytes. (en)
Title	Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing (en)
skos:prefLabel	Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing (en)
skos:notation	RIV/61388963:_____/11:00359988!RIV12-AV0-61388963
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/08/1428), P(GP203/09/P485), Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Koval, Dušan Kašička, Václav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	185666
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/11:00359988
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	capillary zone electrophoresis; capillary isoelectric focusing; glycated hemoglobin HbA1c (en)
http://linked.open.../riv/klicoveSlovo	capillary zone electrophoresis capillary isoelectric focusing glycated hemoglobin HbA1c
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[A079EA3951E4]
http://linked.open...i/riv/nazevZdroje	Analytical Biochemistry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Capillary electrophoresis with multidimensinal detection and its application to separation and characterization of biopolymers Two-dimensional separation techniques for microscale bioanalysis
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	413
http://linked.open...iv/tvurceVysledku	Cottet, H. Kašička, Václav Koval, Dušan
http://linked.open...ain/vavai/riv/wos	000289607900002
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství
issn	0003-2697
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ab.2011.01.048

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