About: New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. This method enables exchange of media containing tested chemicals in various concentrations and simultaneous evaluation of their phytotoxicity and antiviral activity. While using ribavirin as a standard for comparison, phytotoxicity and ability of the acyclic nucleotide analogues (R)-PMPA, PMEA, PMEDAP, and (S)-HPMPC to eliminate ssRNA Turnip yellow mosaic virus (TYMV) were evaluated by this method. Double antibody sandwich ELISA and real-time PCR were used for relative quantification of viral protein and nucleic acid in plants. Ribavirin had the most powerful antiviral effect against TYMV. On the other hand, (R)-PMPA and PMEA had no antiviral effect and almost no phytotoxicity compared to the control. (S)-HPMPC and PMEDAP showed moderate antiviral effect, accompanied by higher phytotoxicity. A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. This method enables exchange of media containing tested chemicals in various concentrations and simultaneous evaluation of their phytotoxicity and antiviral activity. While using ribavirin as a standard for comparison, phytotoxicity and ability of the acyclic nucleotide analogues (R)-PMPA, PMEA, PMEDAP, and (S)-HPMPC to eliminate ssRNA Turnip yellow mosaic virus (TYMV) were evaluated by this method. Double antibody sandwich ELISA and real-time PCR were used for relative quantification of viral protein and nucleic acid in plants. Ribavirin had the most powerful antiviral effect against TYMV. On the other hand, (R)-PMPA and PMEA had no antiviral effect and almost no phytotoxicity compared to the control. (S)-HPMPC and PMEDAP showed moderate antiviral effect, accompanied by higher phytotoxicity. (en)
Title	New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses (en)
skos:prefLabel	New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses (en)
skos:notation	RIV/61388963:_____/10:00350494!RIV11-GA0-61388963
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/09/0707), Z(AV0Z40550506), Z(AV0Z50510513)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Votruba, Ivan Holý, Antonín
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	274688
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/10:00350494
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Brassica; Chemotherapy; Turnip yellow mosaic virus; Ribavirin (en)
http://linked.open.../riv/klicoveSlovo	Brassica Ribavirin Chemotherapy Turnip yellow mosaic virus
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[E199B5E2D1D5]
http://linked.open...i/riv/nazevZdroje	Antiviral Research
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Effect of acyclic nucleosid phosphonates and other antimetabolites against plant viruses
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	88
http://linked.open...iv/tvurceVysledku	Holý, Antonín Pavingerová, Daniela Petrzik, Karel Votruba, Ivan Špak, Josef Špaková, Vlastimila
http://linked.open...ain/vavai/riv/wos	000285660300008
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
issn	0166-3542
number of pages	8 (xsd:int)

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