About: Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity

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About: Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Human serine racemase (hSR) is a cytosolic pyridoxal-5-phosphate dependent enzyme responsible for production of D-serine in the central nervous system. D-Serine acts as an endogenous coagonist of N-methyl-D-aspartate receptor ion channels. Increased levels of D-serine have been linked to amyotrophic lateral sclerosis and Alzheimer’s disease.Here, we present a strategy for the generation and screening of random hSR mutants. From a library of randomly mutated hSR variants, twenty-seven soluble mutants were selected, expressed, and evaluated for enzymatic activity. Taking three carefully characterized mutants as an example, we show how this strategy can be used to pinpoint structurally and functionally important residues. In particular, we identify S84 and P111 as residues crucial for hSR activity and C217 and K221 as residues important for binding of the Mg2+ cofactor. Human serine racemase (hSR) is a cytosolic pyridoxal-5-phosphate dependent enzyme responsible for production of D-serine in the central nervous system. D-Serine acts as an endogenous coagonist of N-methyl-D-aspartate receptor ion channels. Increased levels of D-serine have been linked to amyotrophic lateral sclerosis and Alzheimer’s disease.Here, we present a strategy for the generation and screening of random hSR mutants. From a library of randomly mutated hSR variants, twenty-seven soluble mutants were selected, expressed, and evaluated for enzymatic activity. Taking three carefully characterized mutants as an example, we show how this strategy can be used to pinpoint structurally and functionally important residues. In particular, we identify S84 and P111 as residues crucial for hSR activity and C217 and K221 as residues important for binding of the Mg2+ cofactor. (en)
Title	Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity (en)
skos:prefLabel	Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity Random mutagenesis of human serine racemase reveals residues important for the enzymatic activity (en)
skos:notation	RIV/61388963:_____/10:00343324!RIV11-MSM-61388963
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0508), Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Jirásková, Jana Konvalinka, Jan Hoffman, Hillary Elizabeth
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	283875
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/10:00343324
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	D-serine; serine racemase; random mutagenesis (en)
http://linked.open.../riv/klicoveSlovo	D-serine serine racemase random mutagenesis
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[B22330C2580C]
http://linked.open...i/riv/nazevZdroje	Collection of Czechoslovak Chemical Communications
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	New Antivirals and Antineoplastics
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	75
http://linked.open...iv/tvurceVysledku	Konvalinka, Jan Hoffman, Hillary Elizabeth Jirásková, Jana Zvelebil, M.
http://linked.open...ain/vavai/riv/wos	000274668100004
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství
issn	0010-0765
number of pages	21 (xsd:int)

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