About: Functional expression and characterization of cathepsin B and L from the gut of the tick Ixodes ricinus

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About: Functional expression and characterization of cathepsin B and L from the gut of the tick Ixodes ricinus Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Ticks differ from hematophagous insects in intracellular localization of hemoglobin proteolysis. We have recently described in the hard tick Ixodes ricinus that this process occurs at acidic pH and as is maintained by a machinery of gut-associated cysteine and aspartic proteases similar to blood feeding platyhelmints and nematodes. In this work, we functionally expressed in Pichia pastoris and characterized two components of tick hemoglobinolytic cascade, namely IrCB and IrCL (cysteine proteases of CA family). Using a positional scanning of synthetic combinatorial library we identified the P1–P4 specifities of both enzymes. IrCL displays a strict acidic optimum at pH 4, while IrCB shows broader pH range. Both fusion enzymes were demonstrated to digest bovine hemoglobin and albumin in vitro. Expression profiles of both enzymes were strongly upregulated during blood meal uptake. Ticks differ from hematophagous insects in intracellular localization of hemoglobin proteolysis. We have recently described in the hard tick Ixodes ricinus that this process occurs at acidic pH and as is maintained by a machinery of gut-associated cysteine and aspartic proteases similar to blood feeding platyhelmints and nematodes. In this work, we functionally expressed in Pichia pastoris and characterized two components of tick hemoglobinolytic cascade, namely IrCB and IrCL (cysteine proteases of CA family). Using a positional scanning of synthetic combinatorial library we identified the P1–P4 specifities of both enzymes. IrCL displays a strict acidic optimum at pH 4, while IrCB shows broader pH range. Both fusion enzymes were demonstrated to digest bovine hemoglobin and albumin in vitro. Expression profiles of both enzymes were strongly upregulated during blood meal uptake. (en)
Title	Functional expression and characterization of cathepsin B and L from the gut of the tick Ixodes ricinus Functional expression and characterization of cathepsin B and L from the gut of the tick Ixodes ricinus (en)
skos:prefLabel	Functional expression and characterization of cathepsin B and L from the gut of the tick Ixodes ricinus Functional expression and characterization of cathepsin B and L from the gut of the tick Ixodes ricinus (en)
skos:notation	RIV/61388963:_____/09:00336472!RIV10-MSM-61388963
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA600960910), P(LC06009), Z(AV0Z40550506), Z(AV0Z60220518)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Mareš, Michael Horn, Martin
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	315714
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/09:00336472
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cathepsin B and L; Ixodes ricinus; functionl expresssion (en)
http://linked.open.../riv/klicoveSlovo	cathepsin B and L functionl expresssion Ixodes ricinus
http://linked.open...ontrolniKodProRIV	[CA35C4D5A23C]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for molecular ecology of vectors and pathogens The digestive system of ticks - the target for rational development of vaccine against ticks and tick-borne pathogens
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Horn, Martin Kopáček, Petr Mareš, Michael Sojka, Daniel Franta, Zdeněk Caffrey, C. R. McKerrow, J. H. Dvorak, J. Pěničková, Helena Schneider, E. I.
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni

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