About: Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods     Goto   Sponge   NotDistinct   Permalink

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  • The peptide hydrolysis by GCPII was investigated in detail by carrying out the kinetic analysis of GCPII(E424A) using N-Ac-Asp-Glu as substrate. The crystal structure of GCPII(E424A) in complex with N-Ac-Asp-Glu was determined at 1.70 A resolution. The experimental data were then complemented by the QM/MM calculations which provided detailed information concerning the GCPII reaction mechanism.
  • The peptide hydrolysis by GCPII was investigated in detail by carrying out the kinetic analysis of GCPII(E424A) using N-Ac-Asp-Glu as substrate. The crystal structure of GCPII(E424A) in complex with N-Ac-Asp-Glu was determined at 1.70 A resolution. The experimental data were then complemented by the QM/MM calculations which provided detailed information concerning the GCPII reaction mechanism. (en)
Title
  • Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods
  • Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods (en)
skos:prefLabel
  • Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods
  • Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods (en)
skos:notation
  • RIV/61388963:_____/09:00326498!RIV10-MSM-61388963
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M0508), P(LC512), Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika
  • 19
http://linked.open...vai/riv/dodaniDat
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  • 338184
http://linked.open...ai/riv/idVysledku
  • RIV/61388963:_____/09:00326498
http://linked.open...riv/jazykVysledku
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  • reaction mechanism; QM/MM calculations; glutamate carboxypeptidase II; X-ray crystallography; mutant activity (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [1888AAD5DBAC]
http://linked.open...i/riv/nazevZdroje
  • Biochemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 48
http://linked.open...iv/tvurceVysledku
  • Konvalinka, Jan
  • Rulíšek, Lubomír
  • Klusák, Vojtěch
  • Lubkowski, J.
  • Mlčochová, Petra
  • Plechanovová, Anna
  • Bařinka, C.
http://linked.open...ain/vavai/riv/wos
  • 000266047400012
http://linked.open...n/vavai/riv/zamer
issn
  • 0006-2960
number of pages
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