About: Comparison of specificity and sensitivity of immunochemical and molecular techniques for reliable detection of Erwinia amylovora

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About: Comparison of specificity and sensitivity of immunochemical and molecular techniques for reliable detection of Erwinia amylovora Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Erwinia amylovora byla detekována PCR, IFAS a ELISA v rostlinných vzorcích a v čistých bakteriálních kulturách. Identita všech vzorků byla potvrzena testem Biolog a biologickým testem na hrušních. Citlivost PTA-ELISA v čistých kulturách byla 106-105 CFU/ml, citlivost IFAS a PCR kolem 105-104 CFU/ml. (cs) Erwinia amylovora, the causal agent of fire blight, was detected in plant samples and pure bacterial cultures by means of PCR, IFAS, and ELISA. Using the Biolog system and immature pear fruit assay, identities of all Ea strains were confirmed as the fire blight bacterium. In assays of pure Ea cultures, PTA-ELISA, and both IFAS and PCR were sensitive to concentrations 106-105 and 105-104 CFU/ml, respectively. Erwinia amylovora, the causal agent of fire blight, was detected in plant samples and pure bacterial cultures by means of PCR, IFAS, and ELISA. Using the Biolog system and immature pear fruit assay, identities of all Ea strains were confirmed as the fire blight bacterium. In assays of pure Ea cultures, PTA-ELISA, and both IFAS and PCR were sensitive to concentrations 106-105 and 105-104 CFU/ml, respectively. (en)
Title	Comparison of specificity and sensitivity of immunochemical and molecular techniques for reliable detection of Erwinia amylovora Srovnání specificity a citlivosti imunochemických a molekulárních technik detekce Erwinia amylovora (cs) Comparison of specificity and sensitivity of immunochemical and molecular techniques for reliable detection of Erwinia amylovora (en)
skos:prefLabel	Comparison of specificity and sensitivity of immunochemical and molecular techniques for reliable detection of Erwinia amylovora Srovnání specificity a citlivosti imunochemických a molekulárních technik detekce Erwinia amylovora (cs) Comparison of specificity and sensitivity of immunochemical and molecular techniques for reliable detection of Erwinia amylovora (en)
skos:notation	RIV/61388963:_____/07:00095676!RIV08-AV0-61388963
http://linked.open.../vavai/riv/strany	175;182
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1QS500510558), Z(AV0Z50510513), Z(MSM6046070901), Z(MZE0002700603)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Pytelková, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	414402
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/07:00095676
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Erwinia amylovora; detection (en)
http://linked.open.../riv/klicoveSlovo	detection Erwinia amylovora
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[DB61BF77E4F8]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of phytopathogenes occurrence and its genetic variants
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	52
http://linked.open...iv/tvurceVysledku	Kokošková, B. Mráz, Ivan Hýblová, Jana
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje. Environment friendly systems of plant and stored product protection against harmful organisms aimed at quality and health safety plant products
issn	0015-5632
number of pages	8 (xsd:int)

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