| Attributes | Values |
|---|
| rdf:type
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| Description
| - Z cDNA knihovny ze střev klíšťáka Ornithodoros moubata byly izolovány dva geny kódující inhibitory cysteinových proteáz (Om-cystatin 1 a 2). Exprese obou cystatinů byly zřetelně snížena po nasátí krve. Om-cystatin 1 byl exprimován výhradně ve střevě zatímco Om-cystatin 2 byl přítomen i ve slinných žlázách. Ve střevním obsahu hladových klíšťat byl ve větší míře zastoupen Om-cystatin 2, který byl asociován s tzv. residuálními tělísky - odpadním produktem tvořeným agregovaným hemem. Rekombinantní Om-cystatiny 1 a 2 inhibovaly modelové savčí proteázy papainového typu včetně cathepsinu B a H, výrazně se lišily v inhibici cathepsinu C a vůbec neinhibovaly asparaginylovou endopeptidázu. Naše výsledky ukazují, že sekretované cystatiny mohou hrát různé úlohy podle místa svojí exprese - v regulaci cílových proteáz v trávicím procesu nebo při interakci s hostitelem. (cs)
- Two genes coding for cysteine peptidase inhibitors of the cystatin family (Om-cystatin 1 and 2) were isolated from a gut-specific cDNA library of the soft tick Ornithodoros moubata. Both cystatins were down-regulated after a blood meal. Om-cystatin 1 is mainly expressed in the tick gut, while Om-cystatin 2 mRNA was also found in salivary glands. Om-cystatin 2 was abundantly present in the gut contents of fasting ticks and mainly associated with hemosome-derived residual bodies accumulated in the gut lumen. Recombinant Om-cystatins 1 and 2 inhibited papain-like peptidases, including cathepsin B and H, but differed significantly in their affinity towards cathepsin C and failed to block asparaginyl endopeptidase. Our results suggest that the secreted cystatins are involved in the regulation of multiple proteolytic targets in the tick digestive system and tick-host interaction.
- Two genes coding for cysteine peptidase inhibitors of the cystatin family (Om-cystatin 1 and 2) were isolated from a gut-specific cDNA library of the soft tick Ornithodoros moubata. Both cystatins were down-regulated after a blood meal. Om-cystatin 1 is mainly expressed in the tick gut, while Om-cystatin 2 mRNA was also found in salivary glands. Om-cystatin 2 was abundantly present in the gut contents of fasting ticks and mainly associated with hemosome-derived residual bodies accumulated in the gut lumen. Recombinant Om-cystatins 1 and 2 inhibited papain-like peptidases, including cathepsin B and H, but differed significantly in their affinity towards cathepsin C and failed to block asparaginyl endopeptidase. Our results suggest that the secreted cystatins are involved in the regulation of multiple proteolytic targets in the tick digestive system and tick-host interaction. (en)
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| Title
| - Two secreted cystatins of the soft tick Ornithodoros moubata: differential expression pattern and inhibitory specificity
- Two secreted cystatins of the soft tick Ornithodoros moubata: differential expression pattern and inhibitory specificity (en)
- Dva sekretované cystatiny z klíšťáka Ornithodoros moubata: rozdíly v expresy a inhibiční specifitě (cs)
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| skos:prefLabel
| - Two secreted cystatins of the soft tick Ornithodoros moubata: differential expression pattern and inhibitory specificity
- Two secreted cystatins of the soft tick Ornithodoros moubata: differential expression pattern and inhibitory specificity (en)
- Dva sekretované cystatiny z klíšťáka Ornithodoros moubata: rozdíly v expresy a inhibiční specifitě (cs)
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| skos:notation
| - RIV/61388963:_____/06:00047963!RIV07-AV0-61388963
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA206/06/0865), P(IAA6022307), P(LC06009), Z(AV0Z40550506), Z(AV0Z60220518)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388963:_____/06:00047963
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - cystatin; tick; midgut (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Horn, Martin
- Kopáček, Petr
- Mareš, Michael
- Vancová, Marie
- Sojka, D.
- Franta, Z.
- Grunclová, L.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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