About: Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization of such domains. In this letter we investigate relation between nanodomain affinity of a donor-acceptor (D/A) pair and the detectable nanodomain size/area. We show that probes with high affinity to the liquid-ordered (Lo) phase are required for detecting domain sizes of a few nanometers, and/or domains that occupy a few percent of the bilayer area. A combination of donors and acceptors that prefer different phases is the more favorable approach. For instance, a D/A pair with the distribution constant of donors KD = 5 and acceptors KA = 0.01 can resolve a broad spectrum of nanodomain sizes. On the other hand, currently available donors and acceptors that prefer the same phase, either the liquid-disordered (Ld) or Lo phase, are not so convenient for determining domain sizes <20 nm. Here the detection limits of FRET experiments employing several commonly used D/A pairs have been investigated. Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization of such domains. In this letter we investigate relation between nanodomain affinity of a donor-acceptor (D/A) pair and the detectable nanodomain size/area. We show that probes with high affinity to the liquid-ordered (Lo) phase are required for detecting domain sizes of a few nanometers, and/or domains that occupy a few percent of the bilayer area. A combination of donors and acceptors that prefer different phases is the more favorable approach. For instance, a D/A pair with the distribution constant of donors KD = 5 and acceptors KA = 0.01 can resolve a broad spectrum of nanodomain sizes. On the other hand, currently available donors and acceptors that prefer the same phase, either the liquid-disordered (Ld) or Lo phase, are not so convenient for determining domain sizes <20 nm. Here the detection limits of FRET experiments employing several commonly used D/A pairs have been investigated. (en)
Title	Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes (en)
skos:prefLabel	Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes (en)
skos:notation	RIV/61388955:_____/11:00369083!RIV12-GA0-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP208/10/0376), P(GAP208/10/1090), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Štefl, Martin Šachl, Radek Hof, Martin Humpolíčková, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	209572
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/11:00369083
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Foerster resonance energy transfer; nanodomains; Monte Carlo Setup (en)
http://linked.open.../riv/klicoveSlovo	Foerster resonance energy transfer Monte Carlo Setup nanodomains
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F8EFA1470016]
http://linked.open...i/riv/nazevZdroje	Biophysical Journal
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Interaction of antibacterial peptides with the model membranes and possibility of the prediction of their biological activity Development of New Fluorescence Methods for the Characterization of Pores Formed by Antimicrobial Peptides
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	101
http://linked.open...iv/tvurceVysledku	Hof, Martin Štefl, Martin Šachl, Radek Humpolíčková, Jana Johansson, L. B. A.
http://linked.open...ain/vavai/riv/wos	000297897300002
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	0006-3495
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.bpj.2011.11.001

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