About: Applications of phasor plots to in vitro protein studies

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In a recent article, we described the application of phasor analysis to fluorescence intensity decay data on in vitro samples. As detailed in that article, this method provides researchers with a simple graphical method for viewing lifetime data that can be used to quantify individual components of a mixture as well as to identify excited state reactions. In the current article, we extend the use of in vitro phasor analysis to intrinsic protein fluorescence. We show how alterations in the excited state properties of tryptophan residues are easily visualized using the phasor method. Specifically, we demonstrate that protein-ligand and protein-protein interactions can result in unique shifts in the location of phasor points, indicative of protein conformational changes. Application of the method to a rapid kinetic experiment is also shown. In a recent article, we described the application of phasor analysis to fluorescence intensity decay data on in vitro samples. As detailed in that article, this method provides researchers with a simple graphical method for viewing lifetime data that can be used to quantify individual components of a mixture as well as to identify excited state reactions. In the current article, we extend the use of in vitro phasor analysis to intrinsic protein fluorescence. We show how alterations in the excited state properties of tryptophan residues are easily visualized using the phasor method. Specifically, we demonstrate that protein-ligand and protein-protein interactions can result in unique shifts in the location of phasor points, indicative of protein conformational changes. Application of the method to a rapid kinetic experiment is also shown. (en)
Title	Applications of phasor plots to in vitro protein studies Applications of phasor plots to in vitro protein studies (en)
skos:prefLabel	Applications of phasor plots to in vitro protein studies Applications of phasor plots to in vitro protein studies (en)
skos:notation	RIV/61388955:_____/11:00367712!RIV12-AV0-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06063), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Štefl, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	186899
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/11:00367712
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protein fluorescence; kinetics; lifetimes (en)
http://linked.open.../riv/klicoveSlovo	lifetimes protein fluorescence kinetics
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[3BD003574901]
http://linked.open...i/riv/nazevZdroje	Analytical Biochemistry
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	410
http://linked.open...iv/tvurceVysledku	James, N. G. Jameson, D. M. Ross, J. A. Štefl, Martin
http://linked.open...ain/vavai/riv/wos	000286711300011
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	0003-2697
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ab.2010.11.011

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