About: Nanoshaving of bovine serum albumin films adsorbed on monocrystalline surfaces and interfaces     Goto   Sponge   NotDistinct   Permalink

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  • We apply the ex situ and in situ atomic force microscopy (AFM) nanoshaving technique to investigate the bovine serum albumin (BSA) films on Au(111) and highly oriented pyrolytic graphite (HOPG) surfaces. The both substrates were found to support the BSA films. The section analysis performed before and after the AFM nanoshaving allowed the determination of the film thickness. On Au(111) surface, both ex situ and in situ nanoshaving revealed that the film is formed by strongly denatured BSA molecules, with the average thickness 2.3 0.2 and 2.0 0.2 nm, respectively. On the other hand, the HOPG substrate was found to support less denatured BSA films, with the average film thickness 4.7 0.3 and 5.2 0.3 nm, based on the ex situ and in situ measurements, respectively.
  • We apply the ex situ and in situ atomic force microscopy (AFM) nanoshaving technique to investigate the bovine serum albumin (BSA) films on Au(111) and highly oriented pyrolytic graphite (HOPG) surfaces. The both substrates were found to support the BSA films. The section analysis performed before and after the AFM nanoshaving allowed the determination of the film thickness. On Au(111) surface, both ex situ and in situ nanoshaving revealed that the film is formed by strongly denatured BSA molecules, with the average thickness 2.3 0.2 and 2.0 0.2 nm, respectively. On the other hand, the HOPG substrate was found to support less denatured BSA films, with the average film thickness 4.7 0.3 and 5.2 0.3 nm, based on the ex situ and in situ measurements, respectively. (en)
Title
  • Nanoshaving of bovine serum albumin films adsorbed on monocrystalline surfaces and interfaces
  • Nanoshaving of bovine serum albumin films adsorbed on monocrystalline surfaces and interfaces (en)
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  • Nanoshaving of bovine serum albumin films adsorbed on monocrystalline surfaces and interfaces
  • Nanoshaving of bovine serum albumin films adsorbed on monocrystalline surfaces and interfaces (en)
skos:notation
  • RIV/61388955:_____/11:00362916!RIV12-GA0-61388955
http://linked.open...avai/riv/aktivita
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  • I, P(GA203/08/1157), P(GP203/09/P502), Z(AV0Z40400503)
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  • 9
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  • 214799
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  • RIV/61388955:_____/11:00362916
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  • bovine serum albumin; nanoshaving; proteins (en)
http://linked.open.../riv/klicoveSlovo
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  • CZ - Česká republika
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  • [4E8EE72319B5]
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  • Collection of Czechoslovak Chemical Communications
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  • 76
http://linked.open...iv/tvurceVysledku
  • Gál, Miroslav
  • Hromadová, Magdaléna
  • Kolivoška, Viliam
  • Sokolová, Romana
  • Janda, Pavel
  • Lachmanová, Štěpánka
http://linked.open...ain/vavai/riv/wos
  • 000298004200002
http://linked.open...n/vavai/riv/zamer
issn
  • 0010-0765
number of pages
http://bibframe.org/vocab/doi
  • 10.1135/cccc2011080
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