About: Mass Spectrometric Characterization of Oligomers in Pseudomonas aeruginosa Azurin Solutions

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We have employed laser-induced liquid bead ion desorption mass spectroscopy (LILBID MS) to study the solution behavior of Pseudomonas aeruginosa azurin as well as two mutants and corresponding Re-labeled derivatives containing a Re(CO)3(4,7-dimethyl-1,10-phenanthroline)+ chromophore appended to a surface histidine. LILBID spectra show broad oligomer distributions whose particular patterns depend on the solution composition (pure H2O, 2030 mM NaCl, 20 and 50 mM NaPi or NH4Pi at pH = 7). The distribution maximum shifts to smaller oligomers upon decreasing the azurin concentration and increasing the buffer concentration. Oligomerization is less extensive for native azurin than its mutants. The oligomerization propensities of unlabeled and Re-labeled proteins are generally comparable, and only Re126 shows some preference for the dimer that persists even in highly diluted solutions. We have employed laser-induced liquid bead ion desorption mass spectroscopy (LILBID MS) to study the solution behavior of Pseudomonas aeruginosa azurin as well as two mutants and corresponding Re-labeled derivatives containing a Re(CO)3(4,7-dimethyl-1,10-phenanthroline)+ chromophore appended to a surface histidine. LILBID spectra show broad oligomer distributions whose particular patterns depend on the solution composition (pure H2O, 2030 mM NaCl, 20 and 50 mM NaPi or NH4Pi at pH = 7). The distribution maximum shifts to smaller oligomers upon decreasing the azurin concentration and increasing the buffer concentration. Oligomerization is less extensive for native azurin than its mutants. The oligomerization propensities of unlabeled and Re-labeled proteins are generally comparable, and only Re126 shows some preference for the dimer that persists even in highly diluted solutions. (en)
Title	Mass Spectrometric Characterization of Oligomers in Pseudomonas aeruginosa Azurin Solutions Mass Spectrometric Characterization of Oligomers in Pseudomonas aeruginosa Azurin Solutions (en)
skos:prefLabel	Mass Spectrometric Characterization of Oligomers in Pseudomonas aeruginosa Azurin Solutions Mass Spectrometric Characterization of Oligomers in Pseudomonas aeruginosa Azurin Solutions (en)
skos:notation	RIV/61388955:_____/11:00359153!RIV12-AV0-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06063), P(ME10124), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	16
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Hof, Martin Sýkora, Jan Vlček jr., Antonín
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	210772
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/11:00359153
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	mass spectrometry; oligomers; pseudomonas aeruginosa azurin solutions (en)
http://linked.open.../riv/klicoveSlovo	oligomers pseudomonas aeruginosa azurin solutions mass spectrometry
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[3D050E0E4AC3]
http://linked.open...i/riv/nazevZdroje	Journal of Physical Chemistry B
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Photoinduced Charge Separation in Metalloprotein-Based Systems: Theory and Experiment Flurescence microscopy in biological and medical research
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	115
http://linked.open...iv/tvurceVysledku	Hof, Martin Sýkora, Jan Brutschy, B. Gray, H. B. Sokolová, L. Vlček jr., Antonín Williamson, H.
http://linked.open...ain/vavai/riv/wos	000289697300028
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	1520-6106
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/jp110460k

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