About: Monte Carlo simulation of fluorescence correlation spectroscopy data

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We employed the Monte Carlo simulation methodology to emulate the diffusion of fluorescently labeled particles and understand the source of differences between values of diffusion coefficients (and consequently hydrodynamic radii) of fluorescently labeled nanoparticles measured by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). We used the simulation program developed in our laboratory and studied the diffusion of spherical particles of different sizes, which are labeled on their surface. In this study, we focused on two complicating effects: (i) multiple labeling and (ii) rotational diffusion which affect the fluorescence signal from large particles and hinder the analysis of autocorrelation functions according to simple analytical models. We have shown that the fluorescence fluctuations can be well fitted using the analytical model for small point-like particles, but the obtained parameters deviate in some cases significantly from the real ones. We employed the Monte Carlo simulation methodology to emulate the diffusion of fluorescently labeled particles and understand the source of differences between values of diffusion coefficients (and consequently hydrodynamic radii) of fluorescently labeled nanoparticles measured by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). We used the simulation program developed in our laboratory and studied the diffusion of spherical particles of different sizes, which are labeled on their surface. In this study, we focused on two complicating effects: (i) multiple labeling and (ii) rotational diffusion which affect the fluorescence signal from large particles and hinder the analysis of autocorrelation functions according to simple analytical models. We have shown that the fluorescence fluctuations can be well fitted using the analytical model for small point-like particles, but the obtained parameters deviate in some cases significantly from the real ones. (en)
Title	Monte Carlo simulation of fluorescence correlation spectroscopy data Monte Carlo simulation of fluorescence correlation spectroscopy data (en)
skos:prefLabel	Monte Carlo simulation of fluorescence correlation spectroscopy data Monte Carlo simulation of fluorescence correlation spectroscopy data (en)
skos:notation	RIV/61388955:_____/11:00358508!RIV12-AV0-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/07/0659), P(IAA400400621), P(IAA400500703), P(IAA401110702), P(MEB100907), Z(AV0Z40400503), Z(MSM0021620857)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Hof, Martin Humpolíčková, Jana Benda, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	213692
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/11:00358508
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Monte Carlo Study; fluorescence; spectroscopy (en)
http://linked.open.../riv/klicoveSlovo	Monte Carlo Study fluorescence spectroscopy
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[9545A18CAB5E]
http://linked.open...i/riv/nazevZdroje	Collection of Czechoslovak Chemical Communications
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Multidisciplinary study of aqueous solutions of branched polyelectrolytes Interaction and Structure of Aggregates Firmed in Mixtures of Hydrophobically Modified Water_soluble Polymers and Surfactants Three-dimensional distributions of branched polymers DNA Condensation: Monte-Carlo Simulations, Light Scattering, Fluorescence Correlation Spectroscopy in vitro and in vivo. Associating Amphiphilic Miktoarm and Heteroarm Polyelectrolytes
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	76
http://linked.open...iv/tvurceVysledku	Hof, Martin Procházka, K. Štěpánek, M. Humpolíčková, Jana Košovan, P. Kuldová, J. Limpouchová, Z. Uhlík, F. Benda, Aleš
http://linked.open...ain/vavai/riv/wos	000288340200005
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace Nové molekulární systémy pro pokročilé aplikace prospěšné pro zdraví a šetrné k životnímu prostředí
issn	0010-0765
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1135/cccc2009526

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