About: Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure: methods based on optical microscopy are very convenient for such investigations. We focus on fluorescence correlation spectroscopy (FCS), explain its principles and review its state of the art versions such as 2-focus. Z-scan or scanning FCS, which overcome most artefacts of standard FCS (especially those resulting from the need for an external calibration) making it a reliable and versatile method. FCS is also compared to single particle tracking and fluorescence photobleaching recovery and the applicability and the limitations of the methods are briefly reviewed. We discuss several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition (ionic strength and sugar content), choice of a fluorescent tracer molecule, ... Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure: methods based on optical microscopy are very convenient for such investigations. We focus on fluorescence correlation spectroscopy (FCS), explain its principles and review its state of the art versions such as 2-focus. Z-scan or scanning FCS, which overcome most artefacts of standard FCS (especially those resulting from the need for an external calibration) making it a reliable and versatile method. FCS is also compared to single particle tracking and fluorescence photobleaching recovery and the applicability and the limitations of the methods are briefly reviewed. We discuss several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition (ionic strength and sugar content), choice of a fluorescent tracer molecule, ... (en)
Title	Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy (en)
skos:prefLabel	Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy (en)
skos:notation	RIV/61388955:_____/10:00345815!RIV11-GA0-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/08/0114), P(GEMEM/09/E006), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hof, Martin Macháň, Radek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	268608
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/10:00345815
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	supported lipid bilayer; giant unilamellar vesicle; fluorescence recovery after photobleaching (en)
http://linked.open.../riv/klicoveSlovo	fluorescence recovery after photobleaching giant unilamellar vesicle supported lipid bilayer
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[3842784D9F1A]
http://linked.open...i/riv/nazevZdroje	Biochimica Et Biophysica Acta-Biomembranes
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Specific ion effects for proteins in solutions and related biologically relevant systems. Molecular level physiology and pathology of oxidized phospholipids
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	1798
http://linked.open...iv/tvurceVysledku	Hof, Martin Macháň, Radek
http://linked.open...ain/vavai/riv/wos	000279365000012
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	0005-2736
number of pages	15 (xsd:int)

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