About: Lipopolythiourea/DNA interaction: A biophysical study

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Lipopolythioureas (LPT) are original non cationic systems representing an alternative to cationic lipids. Their high transfection efficiency prompted us to investigate further their biophysical properties, and in particular how thiourea lipids interact with DNA. The interaction of lipopolythiourea with DNA was investigated by fluorescence correlation microscopy (FCS). Influence of the lipid length and nature of the thiourea head on the thiourea/DNA interaction were studied. FCS revealed a strong interaction between lipopolythiourea and DNA, occurring at 1 equivalent of a thiourea lipid by a DNA phosphate group, and leading to a condensed plasmid state. From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells. Lipopolythioureas (LPT) are original non cationic systems representing an alternative to cationic lipids. Their high transfection efficiency prompted us to investigate further their biophysical properties, and in particular how thiourea lipids interact with DNA. The interaction of lipopolythiourea with DNA was investigated by fluorescence correlation microscopy (FCS). Influence of the lipid length and nature of the thiourea head on the thiourea/DNA interaction were studied. FCS revealed a strong interaction between lipopolythiourea and DNA, occurring at 1 equivalent of a thiourea lipid by a DNA phosphate group, and leading to a condensed plasmid state. From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells. (en)
Title	Lipopolythiourea/DNA interaction: A biophysical study Lipopolythiourea/DNA interaction: A biophysical study (en)
skos:prefLabel	Lipopolythiourea/DNA interaction: A biophysical study Lipopolythiourea/DNA interaction: A biophysical study (en)
skos:notation	RIV/61388955:_____/10:00342982!RIV11-MSM-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA400400621), P(LC06063), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	1-3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hof, Martin Kral, Teresa
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	268611
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/10:00342982
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	lipopolythiourea; FCS; plasmid DNA compaction; 31P NMR (en)
http://linked.open.../riv/klicoveSlovo	FCS 31P NMR lipopolythiourea plasmid DNA compaction
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[BF0D39CA4A01]
http://linked.open...i/riv/nazevZdroje	Biophysical Chemistry
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research DNA Condensation: Monte-Carlo Simulations, Light Scattering, Fluorescence Correlation Spectroscopy in vitro and in vivo.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	148
http://linked.open...iv/tvurceVysledku	Hof, Martin Kral, Teresa Mignet, N. Scherman, D. Hersovici, J. Leblond, J.
http://linked.open...ain/vavai/riv/wos	000277743900009
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	0301-4622
number of pages	6 (xsd:int)

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