About: Optical Saturation as a Versatile Tool to Enhance Resolution in Confocal Microscopy

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	One of the most actively developing areas in fluorescence microscopy is the achievement of spatial resolution below Abbe's diffraction limit, which restricts the resolution to several hundreds of nanometers. Most of the approaches in use at this time require a complex optical setup, a difficult mathematical treatment, or usage of dyes with special photophysical properties. In this work, we present a new, to our knowledge, approach in confocal microscopy that enhances the resolution moderately but is both technically and computationally simple. As it is based on the saturation of the transition from the ground state to the first excited state, it is universally applicable with respect to the dye used. One of the most actively developing areas in fluorescence microscopy is the achievement of spatial resolution below Abbe's diffraction limit, which restricts the resolution to several hundreds of nanometers. Most of the approaches in use at this time require a complex optical setup, a difficult mathematical treatment, or usage of dyes with special photophysical properties. In this work, we present a new, to our knowledge, approach in confocal microscopy that enhances the resolution moderately but is both technically and computationally simple. As it is based on the saturation of the transition from the ground state to the first excited state, it is universally applicable with respect to the dye used. (en)
Title	Optical Saturation as a Versatile Tool to Enhance Resolution in Confocal Microscopy Optical Saturation as a Versatile Tool to Enhance Resolution in Confocal Microscopy (en)
skos:prefLabel	Optical Saturation as a Versatile Tool to Enhance Resolution in Confocal Microscopy Optical Saturation as a Versatile Tool to Enhance Resolution in Confocal Microscopy (en)
skos:notation	RIV/61388955:_____/09:00333796!RIV10-MSM-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(KJB400400904), P(LC06063), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Humpolíčková, Jana Benda, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	331831
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/09:00333796
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence microscopy; reconstruction microscopy; cassettes (en)
http://linked.open.../riv/klicoveSlovo	reconstruction microscopy cassettes fluorescence microscopy
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[51610C98A55D]
http://linked.open...i/riv/nazevZdroje	Biophysical Journal
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of modular spectral confocal fluorescence microscope with enhanced spatial resolution, using combined EM-CCD and SPAD detection, and offering special FCS-based point measurement modes. Flurescence microscopy in biological and medical research
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	97
http://linked.open...iv/tvurceVysledku	Humpolíčková, Jana Benda, Aleš Enderlein, J.
http://linked.open...ain/vavai/riv/wos	000271454000029
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	0006-3495
number of pages	7 (xsd:int)

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