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| Description
| - Představujeme robustní využití časově rozlišené fluorescenční korelační spektroskopie (FLCS), nové techniky založené na detekci jednotlivých molekul. Tato metoda určuje zároveň difuzní koeficient a dobu života fluorescence. Ukazujeme, že pomocí FLCS lze rozlišit mezi různými mechanismy kondenzace molekul DNA, které jsou menší než rozlišovací schopnost fluorescenčního mikroskopu. Úspěch nové techniky je založený na tom, že FLCS selektivně charakterizuje difuzní vlastnosti zkondenzovaných molekul v přítomnosti molekul nezkondenzovaných. Zaměrujeme se na mechanismus kondenzace kruhové (10 kbp) a lineární (49 kbp) DNA vyvolané polykationtem sperminem a kationickým surfaktantem CTAB. Ukazujeme, že spermin vyvolává %22all-or-non%22 přechod, zatímco kondenzace způsobená CTAB je postupná. Vyvozené závěry jsou dále podpořeny dvěma standardními technikami – dynamickým rozptylem světla a fluorescenční korelační spektroskopií (cs)
- We introduce a robust utilization of a novel technique based on the detection of single molecules, fluorescence lifetime correlation spectroscopy (FLCS). The method simultaneously determinates diffusion coefficients and fluorescence lifetime. We demonstrate that FLCS can distinguish between different compaction mechanisms of DNA molecules being even smaller than the resolution of a fluorescence microscope. The success of this unique technique is based on the fact that FLCS allows for characterizing the diffusion properties of the condensed forms exclusively in presence of uncondensed DNA molecules. We focus on the condensation mechanism of circular 10 and linear 49 kbp DNA induced by polycation spermine and cationic surfactant CTAB. We show that spermine induces an all-or-none transition, while the condensation with CTAB is gradual. The conclusions drawn are furthermore supported by two standard techniques, dynamic light scattering, and fluorescence correlation spectroscopy.
- We introduce a robust utilization of a novel technique based on the detection of single molecules, fluorescence lifetime correlation spectroscopy (FLCS). The method simultaneously determinates diffusion coefficients and fluorescence lifetime. We demonstrate that FLCS can distinguish between different compaction mechanisms of DNA molecules being even smaller than the resolution of a fluorescence microscope. The success of this unique technique is based on the fact that FLCS allows for characterizing the diffusion properties of the condensed forms exclusively in presence of uncondensed DNA molecules. We focus on the condensation mechanism of circular 10 and linear 49 kbp DNA induced by polycation spermine and cationic surfactant CTAB. We show that spermine induces an all-or-none transition, while the condensation with CTAB is gradual. The conclusions drawn are furthermore supported by two standard techniques, dynamic light scattering, and fluorescence correlation spectroscopy. (en)
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| Title
| - Fluorescence Lifetime Correlation Spectroscopy Reveals Compaction Mechanism of 10 and 49 kbp DNA and Differences between Polycation and Cationic Surfactant
- Fluorescence Lifetime Correlation Spectroscopy Reveals Compaction Mechanism of 10 and 49 kbp DNA and Differences between Polycation and Cationic Surfactant (en)
- Časově rozlišená fluorescenční korelační spektroskopie (FLCS) objasňuje mechanismus kondenzace DNA o délce 10 a 49 kbp a rozdíl mezi kondenzací polykationtem a kationickým surfaktantem (cs)
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| skos:prefLabel
| - Fluorescence Lifetime Correlation Spectroscopy Reveals Compaction Mechanism of 10 and 49 kbp DNA and Differences between Polycation and Cationic Surfactant
- Fluorescence Lifetime Correlation Spectroscopy Reveals Compaction Mechanism of 10 and 49 kbp DNA and Differences between Polycation and Cationic Surfactant (en)
- Časově rozlišená fluorescenční korelační spektroskopie (FLCS) objasňuje mechanismus kondenzace DNA o délce 10 a 49 kbp a rozdíl mezi kondenzací polykationtem a kationickým surfaktantem (cs)
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| skos:notation
| - RIV/61388955:_____/08:00319300!RIV09-AV0-61388955
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(IAA400400621), P(LC06063), Z(AV0Z40400503), Z(MSM0021620857)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61388955:_____/08:00319300
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - fluorescence-correlation spectroscopy; DNA; AFM (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Physical Chemistry B
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hof, Martin
- Procházka, K.
- Štěpánek, M.
- Humpolíčková, Jana
- Benda, Aleš
- Beranová, Lenka
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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