About: Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fluorescence antibunching is a well-known technique for determining the number of independent emitters per molecule or molecular complex. It was rarely applied to autofluorescent proteins due to the necessity of collecting large numbers of fluorescence photons from a single molecule, which is usually impossible to achieve with rather photolabile autofluorescent proteins. Here, we measure fluorescence antibunching on molecules in solution, allowing us to accumulate data over a large number of molecules. We use that method for determining an average stoichiometry of molecular complexes. The proposed method is absolute in the sense that it does not need any calibration or referencing. We develop the necessary theoretical background and check the method on pure dye solutions and on molecular complexes with known stoichiometry. Fluorescence antibunching is a well-known technique for determining the number of independent emitters per molecule or molecular complex. It was rarely applied to autofluorescent proteins due to the necessity of collecting large numbers of fluorescence photons from a single molecule, which is usually impossible to achieve with rather photolabile autofluorescent proteins. Here, we measure fluorescence antibunching on molecules in solution, allowing us to accumulate data over a large number of molecules. We use that method for determining an average stoichiometry of molecular complexes. The proposed method is absolute in the sense that it does not need any calibration or referencing. We develop the necessary theoretical background and check the method on pure dye solutions and on molecular complexes with known stoichiometry. (en) Fluorescenční antibunching je známá metoda pro určování počtu nezávislých emiterů přítomných v molekulárních komplexech. Tato metoda byla ale zřídka aplikována pro studium autofluorescenčních proteinů imobilizovaných na povrchu kvůli jejich nízké fotostabilitě. V této práci jsme měřili fluorescenční antibunching v roztoku a nasbírali tak data z obrovského počtu molekul, což nám umožnilo určit stechiometrii komplexů těchto fotolabilních proteinů. Tato metoda navíc nevyžaduje kalibraci standardem. (cs)
Title	Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes Měření “antibunchingu“ fluorescence v roztoku: cesta k určení stechiometrie molekulovýxch komplexů (cs) Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes (en)
skos:prefLabel	Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes Měření “antibunchingu“ fluorescence v roztoku: cesta k určení stechiometrie molekulovýxch komplexů (cs) Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes (en)
skos:notation	RIV/61388955:_____/07:00089208!RIV08-MSM-61388955
http://linked.open.../vavai/riv/strany	4040;4049
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06063), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Sýkora, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	421348
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/07:00089208
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	exploring fluorescence antibunching; molecular complexes; biophysical methods (en)
http://linked.open.../riv/klicoveSlovo	molecular complexes exploring fluorescence antibunching biophysical methods
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[4E3C98155462]
http://linked.open...i/riv/nazevZdroje	Analytical Chemistry
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	79
http://linked.open...iv/tvurceVysledku	Sýkora, Jan Kaiser, K. Gregor, I. Enderlain, J. Bönigk, W. Schmalzing, G.
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	0003-2700
number of pages	10 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 91 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software