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| rdf:type
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| Description
| - The subject of this report was to investigate headgroup hydration and mobility of two types of mixed lipid vesicles, containing nonionic surfactants; straight chain Brij 98, and polysorbat Tween 80, with the same number of oxyethylene units as Brij, but attached via a sorbitan ring to oleic acid. We used the fluorescence solvent relaxation (SR) approach for the purpose and revealed differences between the two systems. Fluorescent solvent relaxation probes (Prodan, Laurdan, Patman) were found to be localized in mixed lipid vesicles similarly as in pure phospholipid bilayers. The SR parameters (i.e. dynamic Stokes shift, .DELTA..nu., and the time course of the correlation function, C(t)) of such labels are in the same range in both kinds of systems. Each type of the tested surfactants has its own impact on water organization in the bilayer headgroup region probed by Patman. Brij 98 does not modify the solvation characteristics of the dye.
- The subject of this report was to investigate headgroup hydration and mobility of two types of mixed lipid vesicles, containing nonionic surfactants; straight chain Brij 98, and polysorbat Tween 80, with the same number of oxyethylene units as Brij, but attached via a sorbitan ring to oleic acid. We used the fluorescence solvent relaxation (SR) approach for the purpose and revealed differences between the two systems. Fluorescent solvent relaxation probes (Prodan, Laurdan, Patman) were found to be localized in mixed lipid vesicles similarly as in pure phospholipid bilayers. The SR parameters (i.e. dynamic Stokes shift, .DELTA..nu., and the time course of the correlation function, C(t)) of such labels are in the same range in both kinds of systems. Each type of the tested surfactants has its own impact on water organization in the bilayer headgroup region probed by Patman. Brij 98 does not modify the solvation characteristics of the dye. (en)
- Zkoumali jsme hydrataci a mobilitu v oblasti polárních hlav dvojvrstvy pro dva druhy smíšených lipidových vesiklů obsahující neutrální surfactanty, a to Brij 98 s přímým řetězcem a Tween 80 se stejným počtem oxyetylenových jednotek jako Brij, ale se sorbitanem vázaným na oleovou kyselinu. Pro tento účel jsme použili metodu relaxace rozpouštědla. Fluorescenční značky vhodné pro relaxaci rozpouštědla (Prodan, Laurdan, Patman) byly umístěny ve smíšených vesiklech podobně jako ve fosfolipidových dvojvrstvách. Parametry popisujíci relaxaci rozpouštědla (tj. dynamický Stokesův posun a časový průběh korelační funkce C(t)) se pohybují v téměř shodném rozmezí v obou systémech. Každý typ testovaných surfaktantů má nicméně jiný vliv na organizaci vody (značenou Patmanem) v oblasti polárních hlav. Zatímco Brij 98 nijak neovlivňuje solvatační charakteristiky značky, Tween 80 výrazně dehydratuje oblast polárních hlav a snižuje zde mobilitu vody. (cs)
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| Title
| - The use of solvent relaxation technique to investigate headgroup hydration and protein binding of simple and mixed phosphatidylcholine/surfactant bilayer membranes
- Použití metody relaxace rozpouštědla pro mapování hydratace a interakce proteinu v jednoduchých dvojvrstvách a ve dvojvrstvách tvořených směsí fosfatidylcholinu a surfaktantu (cs)
- The use of solvent relaxation technique to investigate headgroup hydration and protein binding of simple and mixed phosphatidylcholine/surfactant bilayer membranes (en)
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| skos:prefLabel
| - The use of solvent relaxation technique to investigate headgroup hydration and protein binding of simple and mixed phosphatidylcholine/surfactant bilayer membranes
- Použití metody relaxace rozpouštědla pro mapování hydratace a interakce proteinu v jednoduchých dvojvrstvách a ve dvojvrstvách tvořených směsí fosfatidylcholinu a surfaktantu (cs)
- The use of solvent relaxation technique to investigate headgroup hydration and protein binding of simple and mixed phosphatidylcholine/surfactant bilayer membranes (en)
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| skos:notation
| - RIV/61388955:_____/07:00082471!RIV08-MSM-61388955
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(1ET400400413), P(GA203/05/2308), P(GD203/05/H001), P(IAA400400503), P(LC06063), Z(AV0Z40400503)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388955:_____/07:00082471
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - time-resolved fluorescence; mixed lipid/surfactant vesicles; Tween 80; Brij 98; interferon alfa-2b (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Biochimica Et Biophysica Acta-Biomembranes
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Hof, Martin
- Sýkora, Jan
- Olžyńska, Agnieszka
- Jelínek, R.
- Cevc, G.
- Rieber, K.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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