About: Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions     Goto   Sponge   NotDistinct   Permalink

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Description
  • Difúze fluorescenčně značeného lipidu byla změřena pro dva modelové systémy, a to pro obří unilamelární vesikly (GUVs) a pro podporované fosfolipidové membrány (SPBs). Pro měření difúzního koeficientu byla použita „Z-scan“ fluorescenční korelační spektroskopie (FCS). Zvláštní pozornost byla věnována tomu, aby se nelišily podmínky měření pro oba modelové systémy a obdržené hodnoty tak mohly být kvantitativně porovnány. Srovnání difúzního koeficientu pro GUVs (D = 7.8 +/- 0.8 mu m(2) s(-1)) a SPBs (D = 3.1+/- 0.3 mu m(2) s(-1)) jasně ukazuje, že difúze lipidů v GUVs je zhruba dvakrát rychlejší než v SPBs. (cs)
  • The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), were determined by fluorescence correlation spectroscopy (FCS) using the Z-scan approach. For the first time, the performed measurements on 1,2-dioleoyl-sn-glycero-3-phosphocholine ( DOPC) bilayers maintain exactly the same experimental conditions for both systems, which allows for a quantitative comparison of lipid diffusion in these two commonly used model membranes. The results obtained revealed that the lipid mobility in free-standing bilayers (D = 7.8 +/- 0.8 mu m(2) s(-1)) is significantly higher than in the bilayer created on the solid support (mica) (D = 3.1 +/- 0.3 mu m(2) s(-1)).
  • The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), were determined by fluorescence correlation spectroscopy (FCS) using the Z-scan approach. For the first time, the performed measurements on 1,2-dioleoyl-sn-glycero-3-phosphocholine ( DOPC) bilayers maintain exactly the same experimental conditions for both systems, which allows for a quantitative comparison of lipid diffusion in these two commonly used model membranes. The results obtained revealed that the lipid mobility in free-standing bilayers (D = 7.8 +/- 0.8 mu m(2) s(-1)) is significantly higher than in the bilayer created on the solid support (mica) (D = 3.1 +/- 0.3 mu m(2) s(-1)). (en)
Title
  • Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions
  • Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions (en)
  • Difúze lipidů v obřích unilamelárních vesiklech je více než dvakrát rychlejší než difúze v podporovaných fosfolipidových membránách za totožných podmínek (cs)
skos:prefLabel
  • Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions
  • Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions (en)
  • Difúze lipidů v obřích unilamelárních vesiklech je více než dvakrát rychlejší než difúze v podporovaných fosfolipidových membránách za totožných podmínek (cs)
skos:notation
  • RIV/61388955:_____/06:00043210!RIV07-AV0-61388955
http://linked.open.../vavai/riv/strany
  • 9096;9099
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/05/2308), P(LC06063), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika
  • 22
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 483398
http://linked.open...ai/riv/idVysledku
  • RIV/61388955:_____/06:00043210
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • fluorescence-correlation spectroscopy; model membranes; molecular friction (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [58E452B61FF0]
http://linked.open...i/riv/nazevZdroje
  • Langmuir
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 22
http://linked.open...iv/tvurceVysledku
  • Hof, Martin
  • Sýkora, Jan
  • Humpolíčková, Jana
  • Benda, Aleš
  • Przybylo, Magdalena
  • Zan, Anna
http://linked.open...n/vavai/riv/zamer
issn
  • 0743-7463
number of pages
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