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| rdf:type
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| Description
| - Liposomy tvořené kladně nabitými lipidy jsou běžně používány pro cílené doručování genů do buněk. Pro aplikaci kationických liposomů je důležité zmapovat jejich povrchové vlastnosti. Z tohoto důvodu byla použita metoda relaxace rozpouštědla, která poskytuje informace o polaritě a viskozitě v mikrookolí fluorescenční sondy. V této práci byly proměřeny liposomy složené z neutrálního lipidu DOPC a kladně nabitého DOTAPu. Závislost relaxačního času , který poskytuje informaci o mobilitě membrány, na molárním zastoupení DOTAPu vykazuje maximum při 30%. Hydratace membrány na úrovni sn1-karbonylů se nemění do tohoto složení liposomů (30% DOTAPu). Koncentrace DOTAPu vyšší než 30% vedou ke zvýšené hydrataci dvojvrstvy. Pro všechny molární koncentrace DOTAPu byly přesně určeny lokalizace jednotlivých fluorescenčních značek pomocí zhášení fluorescence. (cs)
- The biophysical properties of liposome surfaces are critical for interactions between lipid aggregates and macromolecules. Liposomes formed from cationic lipids, commonly used to deliver genes into cells in vitro and in vivo, are an example of such a system. We apply the fluorescence solvent relaxation technique to study the structure and dynamics of fully hydrated liquid crystalline lipid bilayers composed of mixtures of cationic dioleoyltrimethyl-ammoniumpropane (DOTAP) and neutral dioleoylphosphatidylcholine (DOPC). Using three different naphthalene derivatives as fluorescent dyes (Patman, Laurdan and Prodan) allowed different parts of the headgroup region to be probed. Wavelength-dependent parallax quenching measurements resulted in the precise determination of Laurdan and Patman locations within the DOPC bilayer. Acrylamide quenching experiments were used to examine DOTAP-induced dye relocalization. ...
- The biophysical properties of liposome surfaces are critical for interactions between lipid aggregates and macromolecules. Liposomes formed from cationic lipids, commonly used to deliver genes into cells in vitro and in vivo, are an example of such a system. We apply the fluorescence solvent relaxation technique to study the structure and dynamics of fully hydrated liquid crystalline lipid bilayers composed of mixtures of cationic dioleoyltrimethyl-ammoniumpropane (DOTAP) and neutral dioleoylphosphatidylcholine (DOPC). Using three different naphthalene derivatives as fluorescent dyes (Patman, Laurdan and Prodan) allowed different parts of the headgroup region to be probed. Wavelength-dependent parallax quenching measurements resulted in the precise determination of Laurdan and Patman locations within the DOPC bilayer. Acrylamide quenching experiments were used to examine DOTAP-induced dye relocalization. ... (en)
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| Title
| - Headgroup hydration and mobility of DOTAP/DOPC bilayers: A fluorescence solvent relaxation study
- Headgroup hydration and mobility of DOTAP/DOPC bilayers: A fluorescence solvent relaxation study (en)
- Hydratace a mobilita DOTAP/DOPC dvojvrstev: fluorescenční studie metodou relaxací rozpouštědla (cs)
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| skos:prefLabel
| - Headgroup hydration and mobility of DOTAP/DOPC bilayers: A fluorescence solvent relaxation study
- Headgroup hydration and mobility of DOTAP/DOPC bilayers: A fluorescence solvent relaxation study (en)
- Hydratace a mobilita DOTAP/DOPC dvojvrstev: fluorescenční studie metodou relaxací rozpouštědla (cs)
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| skos:notation
| - RIV/61388955:_____/06:00043207!RIV07-AV0-61388955
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(IAA400400503), P(LC06063), Z(AV0Z40400503)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388955:_____/06:00043207
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - excitation wavelength dependence; membrane penetration depth; solvation dynamics (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hof, Martin
- Jurkiewicz, Piotr
- Olžyńska, Agnieszka
- Langner, E.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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