About: Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A new concept based on fluorescence lifetime correlation spectroscopy (FLCS) is presented allowing the simultaneous determination of diffusion coefficients of identical molecules located in different environments. The difference in fluorescence lifetimes, which is the main prerequisite for FLCS, is reached by locating one polution of the dye close to a light-absorbing surface. Since such surfaces quench fluorescence, the fluorescence lifetime of chromophores located close to these surfaces can be tuned in a specific manner. This approach has been demonstrated for a BODIPY-tail-labeled lipid in supported phospholipid bilayers (SPBs) as well as in phospholipid multilayers adsorbed onto solid supports. In particular, the effect of the solid support type on the fluorescence lifetime as well as its dependence on the BODIPY-support distance has been characterized and verified by theoretical considerations based on precise determination of refractive indices of the supports. A new concept based on fluorescence lifetime correlation spectroscopy (FLCS) is presented allowing the simultaneous determination of diffusion coefficients of identical molecules located in different environments. The difference in fluorescence lifetimes, which is the main prerequisite for FLCS, is reached by locating one polution of the dye close to a light-absorbing surface. Since such surfaces quench fluorescence, the fluorescence lifetime of chromophores located close to these surfaces can be tuned in a specific manner. This approach has been demonstrated for a BODIPY-tail-labeled lipid in supported phospholipid bilayers (SPBs) as well as in phospholipid multilayers adsorbed onto solid supports. In particular, the effect of the solid support type on the fluorescence lifetime as well as its dependence on the BODIPY-support distance has been characterized and verified by theoretical considerations based on precise determination of refractive indices of the supports. (en) V této práce se zaměřujeme na nové koncepty výzkumu fluorescenční korelační spektroskopie (FLCS) a na nové perspektivy ve výzkumu fosfolipidových vrstev. (cs)
Title	Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research Fluorescenční korelační spektroskopie: Nové perspektivy ve výzkumu fosfolipidových vrstev (cs) Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research (en)
skos:prefLabel	Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research Fluorescenční korelační spektroskopie: Nové perspektivy ve výzkumu fosfolipidových vrstev (cs) Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research (en)
skos:notation	RIV/61388955:_____/06:00043203!RIV07-AV0-61388955
http://linked.open.../vavai/riv/strany	9580;9585
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/05/2308), P(LC06063), Z(AV0Z10100522), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	23
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Fagulová, Veronika Hof, Martin Benda, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	476067
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/06:00043203
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	spectroscopy; fluorescence; FLCS (en)
http://linked.open.../riv/klicoveSlovo	fluorescence spectroscopy FLCS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C86323A8EEBE]
http://linked.open...i/riv/nazevZdroje	Langmuir
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research Understanding, control, and preparation of self-assembling biomembrane systems at interfaces
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	22
http://linked.open...iv/tvurceVysledku	Hof, Martin Benda, Aleš Deyneka, Alexander Enderlain, J. Fagulová, Veronika
http://linked.open...n/vavai/riv/zamer	Wave and Corpuscular Light Propagation, Optical Materials and Technology Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	0743-7463
number of pages	7 (xsd:int)

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