Attributes | Values |
---|
rdf:type
| |
Description
| - DNA kondenzace je doprovázená dramatickým zmenšením objemu zabíraným molekulou DNA a našla široké využití v non-viriální genové terapii. Metoda fluorescenční korelační spektroskopie, která pomocí analýzy fluktuací fluorescenčního signálu sbíraného z konfokálního objemu poskytuje informace o difůzních vlastnostech sledované látky, se jeví jako vhodná pro sledování kondenzace DNA. V této práci jsou shrnuty výsledky dosažené v laboratoři doc. Hofa v letech 2002-2005. Jsou zmíněny parametry získané pomocí FCS, které nejlépe popisují stupeň DNA kondenzace, vhodná fluorescenční barviva a látky, jež způsobují DNA kondenzaci. (cs)
- DNA condensation is accompanied by dramatic decrease in the volume occupied by DNA molecule and is used in non-viral gene therapy. Method of fluorescence correlation spectroscopy (FCS), which provides the information on the diffusion properties of an object of interest by analyzing the fluctuations of fluorescence signal collected from confocal volume, was found to be suitable for minitoring the process of DNA condensation. In this article we summarize the results achieved in doc. Hof’s group in years 2002-2005. There are mentioned parameters gained by FCS, which are most convenient for describing the DNA condensation, suitable fluorescence dyes and various condensation agents.
- DNA condensation is accompanied by dramatic decrease in the volume occupied by DNA molecule and is used in non-viral gene therapy. Method of fluorescence correlation spectroscopy (FCS), which provides the information on the diffusion properties of an object of interest by analyzing the fluctuations of fluorescence signal collected from confocal volume, was found to be suitable for minitoring the process of DNA condensation. In this article we summarize the results achieved in doc. Hof’s group in years 2002-2005. There are mentioned parameters gained by FCS, which are most convenient for describing the DNA condensation, suitable fluorescence dyes and various condensation agents. (en)
|
Title
| - DNA condensation characterised by fluorescence correlation spectroscopy (FCS)
- DNA condensation characterised by fluorescence correlation spectroscopy (FCS) (en)
- DNA kondenzace charakterizovaná pomocí fluorescenční korelační spektroskopie (FCS) (cs)
|
skos:prefLabel
| - DNA condensation characterised by fluorescence correlation spectroscopy (FCS)
- DNA condensation characterised by fluorescence correlation spectroscopy (FCS) (en)
- DNA kondenzace charakterizovaná pomocí fluorescenční korelační spektroskopie (FCS) (cs)
|
skos:notation
| - RIV/61388955:_____/05:00000699!RIV06-AV0-61388955
|
http://linked.open.../vavai/riv/strany
| |
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(GA203/05/2308), Z(AV0Z40400503)
|
http://linked.open...iv/cisloPeriodika
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/61388955:_____/05:00000699
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - DNA condensation; fluorescence; DNA (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...odStatuVydavatele
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...i/riv/nazevZdroje
| - Cellular & Molecular Biology Letters
|
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...v/svazekPeriodika
| |
http://linked.open...iv/tvurceVysledku
| - Hof, Martin
- Kral, Teresa
- Langner, M.
- Adjimatera, N.
- Blagbrough, I. S.
|
http://linked.open...n/vavai/riv/zamer
| |
issn
| |
number of pages
| |