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Description
  • DNA kondenzace je doprovázená dramatickým zmenšením objemu zabíraným molekulou DNA a našla široké využití v non-viriální genové terapii. Metoda fluorescenční korelační spektroskopie, která pomocí analýzy fluktuací fluorescenčního signálu sbíraného z konfokálního objemu poskytuje informace o difůzních vlastnostech sledované látky, se jeví jako vhodná pro sledování kondenzace DNA. V této práci jsou shrnuty výsledky dosažené v laboratoři doc. Hofa v letech 2002-2005. Jsou zmíněny parametry získané pomocí FCS, které nejlépe popisují stupeň DNA kondenzace, vhodná fluorescenční barviva a látky, jež způsobují DNA kondenzaci. (cs)
  • DNA condensation is accompanied by dramatic decrease in the volume occupied by DNA molecule and is used in non-viral gene therapy. Method of fluorescence correlation spectroscopy (FCS), which provides the information on the diffusion properties of an object of interest by analyzing the fluctuations of fluorescence signal collected from confocal volume, was found to be suitable for minitoring the process of DNA condensation. In this article we summarize the results achieved in doc. Hof’s group in years 2002-2005. There are mentioned parameters gained by FCS, which are most convenient for describing the DNA condensation, suitable fluorescence dyes and various condensation agents.
  • DNA condensation is accompanied by dramatic decrease in the volume occupied by DNA molecule and is used in non-viral gene therapy. Method of fluorescence correlation spectroscopy (FCS), which provides the information on the diffusion properties of an object of interest by analyzing the fluctuations of fluorescence signal collected from confocal volume, was found to be suitable for minitoring the process of DNA condensation. In this article we summarize the results achieved in doc. Hof’s group in years 2002-2005. There are mentioned parameters gained by FCS, which are most convenient for describing the DNA condensation, suitable fluorescence dyes and various condensation agents. (en)
Title
  • DNA condensation characterised by fluorescence correlation spectroscopy (FCS)
  • DNA condensation characterised by fluorescence correlation spectroscopy (FCS) (en)
  • DNA kondenzace charakterizovaná pomocí fluorescenční korelační spektroskopie (FCS) (cs)
skos:prefLabel
  • DNA condensation characterised by fluorescence correlation spectroscopy (FCS)
  • DNA condensation characterised by fluorescence correlation spectroscopy (FCS) (en)
  • DNA kondenzace charakterizovaná pomocí fluorescenční korelační spektroskopie (FCS) (cs)
skos:notation
  • RIV/61388955:_____/05:00000699!RIV06-AV0-61388955
http://linked.open.../vavai/riv/strany
  • 23;25
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/05/2308), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika
  • -
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 518474
http://linked.open...ai/riv/idVysledku
  • RIV/61388955:_____/05:00000699
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • DNA condensation; fluorescence; DNA (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • PL - Polská republika
http://linked.open...ontrolniKodProRIV
  • [2749BA15273C]
http://linked.open...i/riv/nazevZdroje
  • Cellular & Molecular Biology Letters
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 10
http://linked.open...iv/tvurceVysledku
  • Hof, Martin
  • Kral, Teresa
  • Langner, M.
  • Adjimatera, N.
  • Blagbrough, I. S.
http://linked.open...n/vavai/riv/zamer
issn
  • 1425-8153
number of pages
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