| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Vzhledem k tomu, že podporované fosfolipidové dvojvrstvy se zpravidla připravují samovolnou adsorpcí fosfolipidových vesikul na konkrétní povrch, mechanismus této adsopce a faktory, které ji ovlivňují (přítomnost vápenatých iontů, míchání, pufr) byly studovány s využitím elipsometrie. Na příkladu tří různých povrchů (sklo, mica a silica) byl demonstrován vliv interakce povrch-vesikuly pro adsorpci a interakce mezi vesikulami navzájem pro následnou kondenzaci a vytvoření dvojvrstvy. Dále byla věnována pozornost degradaci dioleoylphosphatidylcholinové (DOPC) membrány na mice pomocí fosfolipasy A2 (PLA2). Ukazuje se, že DOPC je rychle degradován, což svědčí proti předsatvě, že by defekty v membráně hrály roli prvotních cílu pro PLA2. V průběha degradace nebyla pozorována změn tloušťky membrány, pouze pokles její hustoty, což je v souladu s výsledky z mikroskopie atomárních sil. (cs)
- Formation of supported membranes by exposure of solid surfaces to phospholipid vesicles is a much-used technique in membrane research. Freshly cleaved mica, because of its superior flatness, is a preferred support and we used ellipsometry to study membrane formation kinetics on mica. Neutral (DOPC) and negatively charged (20% DOPS / 80% DOPC) vesicles were prepared by sonication. Results were compared with membrane formation on silica and glass, and the influence of stirring, buffer, and calcium was assessed. Without calcium, PC vesicles had a low affinity (Kd = 30 µM) for mica, and PS/PC vesicles hardly adsorbed. Addition of calcium promptly caused condensation of the adhering vesicles, with either loss of excess lipid or rapid additional lipid adsorption up to full surface coverage. Vesicle-mica interaction, rather than vesicle-vesicle interaction, seems to cause this condensation process. Membranes on mica proved unstable in Tris-HCl buffer.
- Formation of supported membranes by exposure of solid surfaces to phospholipid vesicles is a much-used technique in membrane research. Freshly cleaved mica, because of its superior flatness, is a preferred support and we used ellipsometry to study membrane formation kinetics on mica. Neutral (DOPC) and negatively charged (20% DOPS / 80% DOPC) vesicles were prepared by sonication. Results were compared with membrane formation on silica and glass, and the influence of stirring, buffer, and calcium was assessed. Without calcium, PC vesicles had a low affinity (Kd = 30 µM) for mica, and PS/PC vesicles hardly adsorbed. Addition of calcium promptly caused condensation of the adhering vesicles, with either loss of excess lipid or rapid additional lipid adsorption up to full surface coverage. Vesicle-mica interaction, rather than vesicle-vesicle interaction, seems to cause this condensation process. Membranes on mica proved unstable in Tris-HCl buffer. (en)
|
| Title
| - Surface-Dependent Transitions during Self-Assembly of Phospholipid Membranes on Mica, Silica, and Glass
- Surface-Dependent Transitions during Self-Assembly of Phospholipid Membranes on Mica, Silica, and Glass (en)
- Povrchově závislé přechody během tvorby fosfolipidové membrány na mice, silice a sklu (cs)
|
| skos:prefLabel
| - Surface-Dependent Transitions during Self-Assembly of Phospholipid Membranes on Mica, Silica, and Glass
- Surface-Dependent Transitions during Self-Assembly of Phospholipid Membranes on Mica, Silica, and Glass (en)
- Povrchově závislé přechody během tvorby fosfolipidové membrány na mice, silice a sklu (cs)
|
| skos:notation
| - RIV/61388955:_____/04:00107576!RIV/2005/AV0/A54005/N
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(LN00A032), Z(AV0Z4040901)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388955:_____/04:00107576
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - silica and glass; phospholipid; surface-dependent (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Hof, Martin
- Beneš, Martin
- Benda, Aleš
- Billy, D.
- Hermens, W. T.
- Speijer, H.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)