About: Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Advanced optical instruments are useful for analysis and manipulation of individual living cells and their internal structures. We have employed Raman microspectroscopic analysis for assessment of algal lipid body (LB) volume in vivo. Some algae contain β-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have detected proportionality between the Raman vibrations of β-carotene and the LB volume. This finding may allow fast acquisition of LB volume approximation valuable e.g. for Raman microspectroscopy assisted cell sorting. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations. Advanced optical instruments are useful for analysis and manipulation of individual living cells and their internal structures. We have employed Raman microspectroscopic analysis for assessment of algal lipid body (LB) volume in vivo. Some algae contain β-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have detected proportionality between the Raman vibrations of β-carotene and the LB volume. This finding may allow fast acquisition of LB volume approximation valuable e.g. for Raman microspectroscopy assisted cell sorting. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations. (en)
Title	Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor (en)
skos:prefLabel	Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor (en)
skos:notation	RIV/60646594:_____/11:#0000013!RIV14-MPO-60646594
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0017/01/01), P(FR-TI1/433), Z(AV0Z20650511)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Trtílek, Martin
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	PSI (Photon Systems Instruments), spol. s r.o.
http://linked.open...dnocenehoVysledku	225646
http://linked.open...ai/riv/idVysledku	RIV/60646594:_____/11:#0000013
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Raman microspectroscopy; microalgae; Trachydiscus minutus; biotechnology; optical tweezers; microfluidics (en)
http://linked.open.../riv/klicoveSlovo	Raman microspectroscopy microfluidics optical tweezers Trachydiscus minutus biotechnology microalgae
http://linked.open...ontrolniKodProRIV	[EF803A3D4BC0]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Bellingham
http://linked.open...i/riv/nazevZdroje	Photonics, Devices, and Systems V (Proceedings of SPIE Vol. 8306)
http://linked.open...in/vavai/riv/obor	BH
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	APPLICATION LABORATORIES OF ADVANCED MICROTECHNOLOGIES AND NANOTECHNOLOGIES *Development of instrumentation and methodology for the selection of photoautotrophic microorganisms for production of higher-generation biofuels
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Bernatová, S. Ježek, J. Nedbal, L. Pilát, Z. Samek, O. Trtílek, Martin Zemánek, P. Šerý, M.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000297582500021
http://linked.open.../riv/zahajeniAkce	2011-08-24 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Research into experimental methods for examination of the physical properties of matter and their application in advanced technologies
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1117/12.912264
http://purl.org/ne...btex#hasPublisher	SPIE
https://schema.org/isbn	978-0-8194-8953-1

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