About: A Photobioreactor System for Precision Cultivation of Photoautotrophic Microorganisms and for High-Content Analysis of Suspension Dynamics

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Small-scale bioreactors for cultivation of photoautotrophic microbes are required for precise characterization of growth parameters of wild-type as well as engineered strains, for their screening, and for optimization of culture conditions. Here, we describe the design and use of a flat-cuvette photobioreactor allowing accurate setting of culture irradiance, temperature, and gas composition that is combined with real-time monitoring by built-in fluorometer and densitometer. The high-power light source based on light emitting diodes generated precise irradiance levels that were programmed via user-designed protocols. The irradiance, temperature, and gas composition can be static or dynamically modulated while optical density and pH can be stabilized in chemostat and turbidostat modes, respectively. We demonstrate that the instrument is able to detect minute variations of growth caused, e.g., by sudden dilution or by circadian rhythms. Small-scale bioreactors for cultivation of photoautotrophic microbes are required for precise characterization of growth parameters of wild-type as well as engineered strains, for their screening, and for optimization of culture conditions. Here, we describe the design and use of a flat-cuvette photobioreactor allowing accurate setting of culture irradiance, temperature, and gas composition that is combined with real-time monitoring by built-in fluorometer and densitometer. The high-power light source based on light emitting diodes generated precise irradiance levels that were programmed via user-designed protocols. The irradiance, temperature, and gas composition can be static or dynamically modulated while optical density and pH can be stabilized in chemostat and turbidostat modes, respectively. We demonstrate that the instrument is able to detect minute variations of growth caused, e.g., by sudden dilution or by circadian rhythms. (en)
Title	A Photobioreactor System for Precision Cultivation of Photoautotrophic Microorganisms and for High-Content Analysis of Suspension Dynamics A Photobioreactor System for Precision Cultivation of Photoautotrophic Microorganisms and for High-Content Analysis of Suspension Dynamics (en)
skos:prefLabel	A Photobioreactor System for Precision Cultivation of Photoautotrophic Microorganisms and for High-Content Analysis of Suspension Dynamics A Photobioreactor System for Precision Cultivation of Photoautotrophic Microorganisms and for High-Content Analysis of Suspension Dynamics (en)
skos:notation	RIV/60646594:_____/08:#0000031!RIV14-GA0-60646594
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0567), P(GA206/05/0894), Z(AV0Z60870520), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Trtílek, Martin Nedbal, Ladislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	PSI (Photon Systems Instruments), spol. s r.o.
http://linked.open...dnocenehoVysledku	354390
http://linked.open...ai/riv/idVysledku	RIV/60646594:_____/08:#0000031
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	algae; cyanobacteria; high-content monitoring; microbial growth; photosynthesis; systems biology (en)
http://linked.open.../riv/klicoveSlovo	systems biology cyanobacteria algae high-content monitoring photosynthesis microbial growth
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[89534107B3AD]
http://linked.open...i/riv/nazevZdroje	Biotechnology and Bioengineering
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for Applied Cybernetics Technical and methodical platform for monitoring and manipulation of biological regulation in plants, algae and cyanobacteria
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	100
http://linked.open...iv/tvurceVysledku	Nedbal, Ladislav Trtílek, Martin Červený, J. Komárek, O. Pakrasi, H. B.
http://linked.open...ain/vavai/riv/wos	000257821800007
http://linked.open...n/vavai/riv/zamer	Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Fyzikální biologie -nové přístupy v biologickém výzkumu
issn	0006-3592
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/bit.21833

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