About: Characterization of rhamnolipids produced by non-pathogenic Acinetobacter and Enterobacter bacteria

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rhamnolipid production by two non-pathogenic bacterial strains Acinetobacter calcoaceticus and Enterobacter asburiae, and established rhamnolipid producer Pseudomonas aeruginosa was investigated. Rhamnolipids were separated from supernatant and further purified by thin-layer chromatography. Mass spectrometry with negative electrospray ionization revealed rhamnolipid homologues varying in chain length and unsaturation. Tandem mass spectrometry identified mono-rhamnolipid and di-rhamnolipid homologues containing one or two 3-hydroxy fatty acids. Several media differing in carbon (sunflower oil, glycerol and sodium citrate), nitrogen (ammonium ions, nitrate) and phosphorus (total content) source, respectively, were tested to obtain enhanced rhamnolipid production. The best production (0.56 g/l) was obtained when nitrate was used as a nitrogen source. Both strains produced rhamnolipids that exhibited excellent emulsification activity with aromatic and aliphatic hydrocarbons and several plant oils. Unlike P. aeruginosa the two strains, i.e. Acinetobacter and Enterobacter, are not pathogenic to humans. Rhamnolipid production by two non-pathogenic bacterial strains Acinetobacter calcoaceticus and Enterobacter asburiae, and established rhamnolipid producer Pseudomonas aeruginosa was investigated. Rhamnolipids were separated from supernatant and further purified by thin-layer chromatography. Mass spectrometry with negative electrospray ionization revealed rhamnolipid homologues varying in chain length and unsaturation. Tandem mass spectrometry identified mono-rhamnolipid and di-rhamnolipid homologues containing one or two 3-hydroxy fatty acids. Several media differing in carbon (sunflower oil, glycerol and sodium citrate), nitrogen (ammonium ions, nitrate) and phosphorus (total content) source, respectively, were tested to obtain enhanced rhamnolipid production. The best production (0.56 g/l) was obtained when nitrate was used as a nitrogen source. Both strains produced rhamnolipids that exhibited excellent emulsification activity with aromatic and aliphatic hydrocarbons and several plant oils. Unlike P. aeruginosa the two strains, i.e. Acinetobacter and Enterobacter, are not pathogenic to humans. (en)
Title	Characterization of rhamnolipids produced by non-pathogenic Acinetobacter and Enterobacter bacteria Characterization of rhamnolipids produced by non-pathogenic Acinetobacter and Enterobacter bacteria (en)
skos:prefLabel	Characterization of rhamnolipids produced by non-pathogenic Acinetobacter and Enterobacter bacteria Characterization of rhamnolipids produced by non-pathogenic Acinetobacter and Enterobacter bacteria (en)
skos:notation	RIV/60461373:22810/13:43894966!RIV15-GA0-22810___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP503/11/0215)
http://linked.open...iv/cisloPeriodika	prosinec 2012
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Chudoba, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Rektorát
http://linked.open...dnocenehoVysledku	65096
http://linked.open...ai/riv/idVysledku	RIV/60461373:22810/13:43894966
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Rhamnolipids; Enterobacter asburiae; Acinetobacter calcoaceticus; Pseudomonas aeruginosa (en)
http://linked.open.../riv/klicoveSlovo	Pseudomonas aeruginosa Acinetobacter calcoaceticus Enterobacter asburiae Rhamnolipids
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[548FBB7B4A81]
http://linked.open...i/riv/nazevZdroje	Bioresource Technology
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of the composition of triacylglycerols and other lipids in nutritionally important microbial oils and fats.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	130
http://linked.open...iv/tvurceVysledku	Schreiberová, Olga Sigler, Karel Řezanka, Tomáš Chudoba, Josef Masák, Jan Hošková, Miriam Ježdík, Richard
http://linked.open...ain/vavai/riv/wos	000316032300068
issn	0960-8524
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.biortech.2012.12.085
http://localhost/t...ganizacniJednotka	22810

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