About: Detection of immunoglobulins in a laser induced fluorescence system utilizing polydimethysiloxane microchips with advanced surface and optical properties

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About: Detection of immunoglobulins in a laser induced fluorescence system utilizing polydimethysiloxane microchips with advanced surface and optical properties Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We developed an automated laser induced fluorescence system utilizing microfluidic chips for detection and quantification of immunoglobulins. Microchips were fabricated from polydimethysiloxane (PDMS) using the so-called %22prepolymerization technique.%22 The microchip structure helped minimize the effects of PDMS autofluorescence and light scattering. Furthermore, a thin and uniform PDMS layer forming the top of the microchip enabled proper focusing and collection of the excitation beam and the emitted fluorescence, respectively. The developed system was tested for the detection of mouse immunoglobulins. The capturing antibodies were immobilized on internal microchannel walls in the form of a polyelectrolyte. We clearly show that this immobilization technique, if correctly realized, gives results with high reproducibility. After sample incubation and washing, secondary antibodies labeled by fluorescein isothiocyanate were introduced into microchannels to build a detectable complex. We developed an automated laser induced fluorescence system utilizing microfluidic chips for detection and quantification of immunoglobulins. Microchips were fabricated from polydimethysiloxane (PDMS) using the so-called %22prepolymerization technique.%22 The microchip structure helped minimize the effects of PDMS autofluorescence and light scattering. Furthermore, a thin and uniform PDMS layer forming the top of the microchip enabled proper focusing and collection of the excitation beam and the emitted fluorescence, respectively. The developed system was tested for the detection of mouse immunoglobulins. The capturing antibodies were immobilized on internal microchannel walls in the form of a polyelectrolyte. We clearly show that this immobilization technique, if correctly realized, gives results with high reproducibility. After sample incubation and washing, secondary antibodies labeled by fluorescein isothiocyanate were introduced into microchannels to build a detectable complex. (en)
Title	Detection of immunoglobulins in a laser induced fluorescence system utilizing polydimethysiloxane microchips with advanced surface and optical properties Detection of immunoglobulins in a laser induced fluorescence system utilizing polydimethysiloxane microchips with advanced surface and optical properties (en)
skos:prefLabel	Detection of immunoglobulins in a laser induced fluorescence system utilizing polydimethysiloxane microchips with advanced surface and optical properties Detection of immunoglobulins in a laser induced fluorescence system utilizing polydimethysiloxane microchips with advanced surface and optical properties (en)
skos:notation	RIV/60461373:22340/11:43891857!RIV12-MSM-22340___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GD104/08/H055), P(KAN208240651), S, Z(MSM6046137306)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Schrott, Walter Přibyl, Michal Nebyla, Marek Šnita, Dalimil
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská
http://linked.open...dnocenehoVysledku	193711
http://linked.open...ai/riv/idVysledku	RIV/60461373:22340/11:43891857
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	LENGTH; SEPARATIONS; POLYDIMETHYLSILOXANE; CHIP; DEVICES; POLY(DIMETHYLSILOXANE); MICROFLUIDIC CHANNELS; CAPILLARY-ELECTROPHORESIS (en)
http://linked.open.../riv/klicoveSlovo	CAPILLARY-ELECTROPHORESIS CHIP DEVICES LENGTH MICROFLUIDIC CHANNELS POLY(DIMETHYLSILOXANE) POLYDIMETHYLSILOXANE SEPARATIONS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[190652A435DD]
http://linked.open...i/riv/nazevZdroje	Biomicrofluidics
http://linked.open...in/vavai/riv/obor	CI
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Transport and reaction processes in complex multiphase systems Study of interactions of biological macromolecules and nanolayers with a view to research of polymer microfluidic biosensors and therapeutic nanoparticles
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	5
http://linked.open...iv/tvurceVysledku	Šnita, Dalimil Nebyla, Marek Přibyl, Michal Schrott, Walter
http://linked.open...ain/vavai/riv/wos	000289148400019
http://linked.open...n/vavai/riv/zamer	Reakčně-transportní chemicko-inženýrské systémy a jejich dynamické chování, modelování a řízení chemických a biotechnologických procesů
issn	1932-1058
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1063/1.3553006
http://localhost/t...ganizacniJednotka	22340

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