About: Detection of bioaffinity complexes in microchannels by fluorescence techniques.

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Práce je zaměřena na výzkum vícekanaálového mikrofluidního zařízení pro bioanalýzu. Je posána technika přípravy mkročipu z PDMS. Je navržena sendvičová metoda stanovení feritinu v mikrokanálku a jsou uskutečněny validační experimenty. (cs) This work deals with multichannel microfluidic systems intended for bioapplications, particularly for medical diagnostics. Microfluidic systems consist of several parallel channels with small characteristic dimensions (10-100 μm). These devices are fabricated from polystyrene (PS) and polydimethylsiloxane (PDMS) by a combination of microfabrication techniques ? UV photolithography, micromilling, thermal bonding, galvanic metallization, casting etc. In the experiments, the biological receptor is immobilized on the microchannel walls by a passive sorption. The blocking reagent 1 % BSA is used to cover empty regions on the walls. Then, aqueous solutions of ligands and detection reagents with a fluorescent marker are subsequently introduced in the channels. Finally, a solution of a detergent is used to break nonspecific bonds among the biomolecules. The main advantage of the used experimental arrangement is that the reaction time of each reaction step in this experimental arrangement is not longer t This work deals with multichannel microfluidic systems intended for bioapplications, particularly for medical diagnostics. Microfluidic systems consist of several parallel channels with small characteristic dimensions (10-100 μm). These devices are fabricated from polystyrene (PS) and polydimethylsiloxane (PDMS) by a combination of microfabrication techniques ? UV photolithography, micromilling, thermal bonding, galvanic metallization, casting etc. In the experiments, the biological receptor is immobilized on the microchannel walls by a passive sorption. The blocking reagent 1 % BSA is used to cover empty regions on the walls. Then, aqueous solutions of ligands and detection reagents with a fluorescent marker are subsequently introduced in the channels. Finally, a solution of a detergent is used to break nonspecific bonds among the biomolecules. The main advantage of the used experimental arrangement is that the reaction time of each reaction step in this experimental arrangement is not longer t (en)
Title	Detection of bioaffinity complexes in microchannels by fluorescence techniques. Detection of bioaffinity complexes in microchannels by fluorescence techniques. (en) Detekce bioafinitních komplexů v mikrokanále fluorescenčními technikami (cs)
skos:prefLabel	Detection of bioaffinity complexes in microchannels by fluorescence techniques. Detection of bioaffinity complexes in microchannels by fluorescence techniques. (en) Detekce bioafinitních komplexů v mikrokanále fluorescenčními technikami (cs)
skos:notation	RIV/60461373:22340/08:00020219!RIV09-AV0-22340___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD104/08/H055), P(KAN208240651), Z(MSM6046137306)
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Slouka, Zdeněk Schrott, Walter Přibyl, Michal Meisterová, Lucie
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská
http://linked.open...dnocenehoVysledku	362800
http://linked.open...ai/riv/idVysledku	RIV/60461373:22340/08:00020219
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	immunoassay; microfluidic device; fluorescence; microchip; biosensor; optical detection; microfabrication (en)
http://linked.open.../riv/klicoveSlovo	microchip microfabrication microfluidic device optical detection fluorescence biosensor immunoassay
http://linked.open...ontrolniKodProRIV	[7ACD9E48FE15]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	CD-ROM of Full Texts CHISA 2008
http://linked.open...in/vavai/riv/obor	CI
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Transport and reaction processes in complex multiphase systems Study of interactions of biological macromolecules and nanolayers with a view to research of polymer microfluidic biosensors and therapeutic nanoparticles
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Slouka, Zdeněk Přibyl, Michal Schrott, Walter Pecháčková, Lucie
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2008-08-24 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Reakčně-transportní chemicko-inženýrské systémy a jejich dynamické chování, modelování a řízení chemických a biotechnologických procesů
number of pages	8 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Process Engineering Publisher
https://schema.org/isbn	978-80-02-02047-9
http://localhost/t...ganizacniJednotka	22340

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