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rdf:type
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Description
| - Microfluidic protein chips are devices based on the complementary reaction between a ligand and a receptor. The characteristic size of the devices is in order of microns. This work is focused on study of bioaffinity assays in a polystyrene microchip. Two different arrangements for quantification of human immunoglobulins (hIgG) are described. In both cases, the assays are non-competitive and heterogeneous with protein A (PA) immobilized on polystyrene by a passive sorption as receptors. PA binds the Fc region of human immunoglobulins (ligand). In the one step assay, immobilized PA binds fluorescently labeled hIgG; in the sandwich assay, hIgG is bound as a primary ligand and then a fluorescently labeled anti-human goat IgG as the detection antibody. A mathematical model of the incubation phase of the one-step assay is focused on determination of the kinetic constants of heterogeneous PA-hIgG binding in the polystyrene microfluidic chip.
- Microfluidic protein chips are devices based on the complementary reaction between a ligand and a receptor. The characteristic size of the devices is in order of microns. This work is focused on study of bioaffinity assays in a polystyrene microchip. Two different arrangements for quantification of human immunoglobulins (hIgG) are described. In both cases, the assays are non-competitive and heterogeneous with protein A (PA) immobilized on polystyrene by a passive sorption as receptors. PA binds the Fc region of human immunoglobulins (ligand). In the one step assay, immobilized PA binds fluorescently labeled hIgG; in the sandwich assay, hIgG is bound as a primary ligand and then a fluorescently labeled anti-human goat IgG as the detection antibody. A mathematical model of the incubation phase of the one-step assay is focused on determination of the kinetic constants of heterogeneous PA-hIgG binding in the polystyrene microfluidic chip. (en)
- Byly studovány dvě experimentální uspořádání pro kvantifikaci lidských protilátek ze vzorku. Uspořádání byly nekompetitivní a heterogenní. Receptorem byl protein A a ligandem lidská protilátka. Detekce vzniklého komplexu byla prováděna fluorescenčně. Pomocí matematického modelu byly odhadnuty hodnoty kinetické a rovnovážné konstanty systémů Protein A - IgG. (cs)
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Title
| - SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP
- SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP (en)
- Sendvičová bioanalýza v polystyrénovém mikročipu (cs)
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skos:prefLabel
| - SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP
- SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP (en)
- Sendvičová bioanalýza v polystyrénovém mikročipu (cs)
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skos:notation
| - RIV/60461373:22340/07:00018093!RIV08-AV0-22340___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GD104/03/H141), P(KAN208240651), Z(MSM6046137306)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22340/07:00018093
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - immunoassay; antibody; antigen; receptor; ligand; binding constant; human IgG; protein A; microdevice; immunosensor; biosensor; microchip; specific reaction rate (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
| - Tatranské Matliare, Slovakia
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - Proceedings of 34th International Conference of Slovak Society of Chemical Engineering, 21.-25.5.2007, Tatranské Matliare, Slovenská republika
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Přibyl, Michal
- Marek, Miloš
- Štěpánek, Jakub
- Pecháčková, Lucie
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - Slovenská společnost chemického inženýrství
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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