About: Microfluidic chip for fast bioassays-evaluation of binding parameters

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Sedmikanálový polystyrenový mikročip byl vyroben mikroobráběním. V čipu byly provedeny jednokroková a sendvičová bioafinitní reakce pro určení koncentrace protilátek ve vzorku. Doba inkubační fáze trvala 5 minut. Byl vytvořen matematický model inkubační fáze. Optimalizačním výpočtem byly určeny kinetická konstanta tvorby imunoafinitního systému protein A - IgG (kon=5.5 m3 mol-1 s-1) a rozvnovážná disociační konstanta (Kd=3x10-6 mol m-3). (cs) A seven channel polystyrene (PS) microchip has been constructed using a micromilling machine and a high-temperature assembling. Protein A (PA) has been immobilized by a passive sorption on the microchannel walls. Two bioaffinity assays with human immunoglobulin G (hIgG) as a ligand have been carried out. (i) PA as the receptor and fluorescently labeled hIgG (FITC-hIgG) as the ligand, (ii) PA as the receptor with hIgG as the quantified ligand and fluorescently labeled goat anti-human IgG (FITC-gIgG) as the secondary ligand. One incubation step of the assays took only 5 min instead of hours typical for enzyme-linked immunosorbent assay applications. Calibration curves of the dependence of a fluorescence signal on the hIgG concentration in a sample have been obtained in one step due to a parallel arrangement of microchannels. A mathematical model of the PA-FITC-hIgG complex formation in the chip has been developed. The values of the kinetic constant of the PA-FITC-hIgG binding (kon=5.5 m3 mol-1 s-1) A seven channel polystyrene (PS) microchip has been constructed using a micromilling machine and a high-temperature assembling. Protein A (PA) has been immobilized by a passive sorption on the microchannel walls. Two bioaffinity assays with human immunoglobulin G (hIgG) as a ligand have been carried out. (i) PA as the receptor and fluorescently labeled hIgG (FITC-hIgG) as the ligand, (ii) PA as the receptor with hIgG as the quantified ligand and fluorescently labeled goat anti-human IgG (FITC-gIgG) as the secondary ligand. One incubation step of the assays took only 5 min instead of hours typical for enzyme-linked immunosorbent assay applications. Calibration curves of the dependence of a fluorescence signal on the hIgG concentration in a sample have been obtained in one step due to a parallel arrangement of microchannels. A mathematical model of the PA-FITC-hIgG complex formation in the chip has been developed. The values of the kinetic constant of the PA-FITC-hIgG binding (kon=5.5 m3 mol-1 s-1) (en)
Title	Microfluidic chip for fast bioassays-evaluation of binding parameters Microfluidic chip for fast bioassays-evaluation of binding parameters (en) Mikrofluidní čip pro bioanalýzu - určení vazných parametrů (cs)
skos:prefLabel	Microfluidic chip for fast bioassays-evaluation of binding parameters Microfluidic chip for fast bioassays-evaluation of binding parameters (en) Mikrofluidní čip pro bioanalýzu - určení vazných parametrů (cs)
skos:notation	RIV/60461373:22340/07:00018089!RIV08-AV0-22340___
http://linked.open.../vavai/riv/strany	024101-024101
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(KAN208240651), Z(MSM6046137306)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Marek, Miloš Přibyl, Michal Šnita, Dalimil
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská
http://linked.open...dnocenehoVysledku	433704
http://linked.open...ai/riv/idVysledku	RIV/60461373:22340/07:00018089
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Protein A; IgG; microfluidic chip; bioassay (en)
http://linked.open.../riv/klicoveSlovo	bioassay IgG Protein A microfluidic chip
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F428BE6CB07C]
http://linked.open...i/riv/nazevZdroje	Biomicrofluidics
http://linked.open...in/vavai/riv/obor	CI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of interactions of biological macromolecules and nanolayers with a view to research of polymer microfluidic biosensors and therapeutic nanoparticles
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	1
http://linked.open...iv/tvurceVysledku	Šnita, Dalimil Přibyl, Michal Marek, Miloš Štěpánek, Jakub
http://linked.open...n/vavai/riv/zamer	Reakčně-transportní chemicko-inženýrské systémy a jejich dynamické chování, modelování a řízení chemických a biotechnologických procesů
issn	1932-1058
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	22340

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