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rdf:type
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Description
| - Práce je zaměřena na vývoj mikrofluidních zařízení pro kvantitativní stanovení IgG. Zařízení je vyrobeno technikou mikroobrábění. Experimentální data dokazují, že kvantitativní detekce IgG v navrženém multikanálovém mikročipu je možná během několika minut. (cs)
- The work is focused on development of microfluidic polystyrene chips for detection of biological agents. The microfluidic chips are constructed with the use of the micro-milling technology. The interaction among the immobilized bio-molecules and the complementary molecules from an analyte is the principle of the microfluidic chip. Here, the immobilized Protein A (PA) binds the complementary human immunoglobulin G (IgG) from the liquid sample and the protein-protein complex is formed. Because the immunoglobulins are fluorescently labeled, the observed fluorescence signal corresponds with the concentration of the biological macromolecule in the sample. Specificity of the interaction PA - human IgG is tested in blind experiments. Bovine serum albumin (BSA) is used as the blocking agent and Tris buffer with detergent Tween disintegrates the non-specific bonds. The experimental results demonstrate that the immunoglobulins can be quantitatively detected in the proposed multichannel microchip within few
- The work is focused on development of microfluidic polystyrene chips for detection of biological agents. The microfluidic chips are constructed with the use of the micro-milling technology. The interaction among the immobilized bio-molecules and the complementary molecules from an analyte is the principle of the microfluidic chip. Here, the immobilized Protein A (PA) binds the complementary human immunoglobulin G (IgG) from the liquid sample and the protein-protein complex is formed. Because the immunoglobulins are fluorescently labeled, the observed fluorescence signal corresponds with the concentration of the biological macromolecule in the sample. Specificity of the interaction PA - human IgG is tested in blind experiments. Bovine serum albumin (BSA) is used as the blocking agent and Tris buffer with detergent Tween disintegrates the non-specific bonds. The experimental results demonstrate that the immunoglobulins can be quantitatively detected in the proposed multichannel microchip within few (en)
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Title
| - HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS
- HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS (en)
- Heteogenní imunoanalýza v polystyrenovém mikročipu (cs)
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skos:prefLabel
| - HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS
- HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS (en)
- Heteogenní imunoanalýza v polystyrenovém mikročipu (cs)
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skos:notation
| - RIV/60461373:22340/06:00016656!RIV07-MSM-22340___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GP104/03/D006), Z(MSM6046137306)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22340/06:00016656
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - heterogeneous immunoassay; antigen; antibody; microchip; microdevice; microsensor; protein A; human IgG (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - 33rd International Conference of SSCHE, Tatranské Matliare
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Přibyl, Michal
- Marek, Miloš
- Štěpánek, Jakub
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - Slovak Society of Chemical Engineering
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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