| Attributes | Values |
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| rdf:type
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| Description
| - Tato práce je zaměřena na vývoj experimentálního čipu pro heterogenní imunoanalýzu. Polystyren byl použit jako substrát pro výrobu čipu. Při výrobě byly použity techniky mikrofrézování a slinování za tepla. Byly studovány dvě metody detekce biologických molekul - lidských IgG: (i) jednokroková ProteinA-lidský IgG-FITC a (ii) sendvičová ProteinA - lidský IgG - kozí IgG-FITC. Byla vyvinuta imobiizační procedura a oběma technikami byly estrojeny kalibrační křivky závislosti fluorescenčního signálu na koncentraci IgG ve vzorku. (cs)
- This work is focused on development of an experimental microchip for heterogeneous immunoassay. Polystyrene substrate is used as the material for fabrication of the microfluidic chip. Techniques of hot embossing and micro-milling are employed in the fabrication. The central part of the chip contains immobilized protein molecules with biological affinity (Protein A - PA). Two procedures of PA immobilization have been studied: (i) passive sorption and (ii) covalent crosslinking of protein layers. It has been found that both these methods are proper. In the one-step assay, liquid samples containing the complementary molecules (human immunoglobulins FITC labeled - hIgG) are dosed in the place of the immobilized layer through the inlet/outlet microfluidic channels. A sandwich (multi-step) system Protein A - human immunoglobulins - goat immunoglobulins (FITC labeled - gIgG) is also studied. After washing out, the fluorescence intensity in microchannels is proportional to the amount of the formed
- This work is focused on development of an experimental microchip for heterogeneous immunoassay. Polystyrene substrate is used as the material for fabrication of the microfluidic chip. Techniques of hot embossing and micro-milling are employed in the fabrication. The central part of the chip contains immobilized protein molecules with biological affinity (Protein A - PA). Two procedures of PA immobilization have been studied: (i) passive sorption and (ii) covalent crosslinking of protein layers. It has been found that both these methods are proper. In the one-step assay, liquid samples containing the complementary molecules (human immunoglobulins FITC labeled - hIgG) are dosed in the place of the immobilized layer through the inlet/outlet microfluidic channels. A sandwich (multi-step) system Protein A - human immunoglobulins - goat immunoglobulins (FITC labeled - gIgG) is also studied. After washing out, the fluorescence intensity in microchannels is proportional to the amount of the formed (en)
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| Title
| - Development of a microfluidic chip for heterogeneous immunoassay
- Vývoj mikrofluidního čipu pro heterogenní imunoanalýzu (cs)
- Development of a microfluidic chip for heterogeneous immunoassay (en)
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| skos:prefLabel
| - Development of a microfluidic chip for heterogeneous immunoassay
- Vývoj mikrofluidního čipu pro heterogenní imunoanalýzu (cs)
- Development of a microfluidic chip for heterogeneous immunoassay (en)
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| skos:notation
| - RIV/60461373:22340/06:00016651!RIV07-MSM-22340___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GP104/03/D006), Z(MSM6046137306)
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22340/06:00016651
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - heterogeneous immunoassay; microchip fabrication; antibody; Protein A; onestep assay; sandwich assay (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Sborník 17th International Congress of Chemical and Process Engineering
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Šnita, Dalimil
- Přibyl, Michal
- Schrott, Walter
- Štěpánek, Jakub
- Pecháčková, Lucie
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| http://linked.open...vavai/riv/typAkce
| |
| http://linked.open.../riv/zahajeniAkce
| |
| http://linked.open...n/vavai/riv/zamer
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Czech Society of Chemical Engineering
|
| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
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