About: Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Soluble species of phosphorus, sulfur, selenium and eight metals (Mn, Fe, Co, Ni, Cu, Zn, Mo and Cd) in soybean flour and common bean seeds were investigated by size exclusion chromatography (SEC) and ICP-MS. Samples were extracted by 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5). Fractionation of sample extracts by preparative scale SEC was accomplished using Fractogel EMD BioSEC column (600 16 mm) and 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5) as a mobile phase (flow rate 2 ml min-1). 2 ml of sample were injected. Contents of elements in chromatographic fractions were determined by AAS, ICP-AES and ICP-MS. The elution profiles of P, Fe, Co, Ni, Cu, Zn and Mo in both samples were similar. Main species of Co, Ni, Cu, Zn and Mo were found in low molecular weight region (2 to 5 kDa), whereas Fe is predominantly bound to high molecular weight compounds (180 kDa). Dominant phosphorus fraction was detected in medium molecular weight region (10 to 30 kDa) and the other one in low molecular weight regio Soluble species of phosphorus, sulfur, selenium and eight metals (Mn, Fe, Co, Ni, Cu, Zn, Mo and Cd) in soybean flour and common bean seeds were investigated by size exclusion chromatography (SEC) and ICP-MS. Samples were extracted by 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5). Fractionation of sample extracts by preparative scale SEC was accomplished using Fractogel EMD BioSEC column (600 16 mm) and 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5) as a mobile phase (flow rate 2 ml min-1). 2 ml of sample were injected. Contents of elements in chromatographic fractions were determined by AAS, ICP-AES and ICP-MS. The elution profiles of P, Fe, Co, Ni, Cu, Zn and Mo in both samples were similar. Main species of Co, Ni, Cu, Zn and Mo were found in low molecular weight region (2 to 5 kDa), whereas Fe is predominantly bound to high molecular weight compounds (180 kDa). Dominant phosphorus fraction was detected in medium molecular weight region (10 to 30 kDa) and the other one in low molecular weight regio (en)
Title	Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS (en)
skos:prefLabel	Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS (en)
skos:notation	RIV/60461373:22340/02:00007175!RIV/2004/GA0/223404/N
http://linked.open.../vavai/riv/strany	261-273
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA525/00/0581)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Mestek, Oto Kvasnička, František Koplík, Richard Suchánek, Miloslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská
http://linked.open...dnocenehoVysledku	646512
http://linked.open...ai/riv/idVysledku	RIV/60461373:22340/02:00007175
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Inductively coupled plasma mass spectrometry; Size exclusion chromatography; Phosphorus; Trace elements (en)
http://linked.open.../riv/klicoveSlovo	Inductively coupled plasma mass spectrometry Size exclusion chromatography Phosphorus Trace elements
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[43028B124721]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Speciation analysis of trace elements in foods
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	770
http://linked.open...iv/tvurceVysledku	Koplík, Richard Mestek, Oto Kvasnička, František Suchánek, Miloslav Fingerová, Hana Cincibuchová, Jana
issn	1570-0232
number of pages	13 (xsd:int)
http://localhost/t...ganizacniJednotka	22340

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