About: Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge

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About: Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The cross-reactivity of antibodies employed within immunochemistry-based analytical methods may lead overestimation of the results. Under certain conditions, specifically when controlling mycotoxin maximum limits serious problems can be encountered. Not only the structurally related mycotoxins, such as their masked (conjugated) forms, but also the unidentified matrix components are responsible for concentration overestimation of respective target analytes. The cross-reactivity phenomenon may also pose a risk of miss-interpretation of the proficiency tests results, when the assigned value becomes influenced by over-estimated results reported by users of immunochemical tests. In this paper, the current state of the knowledge on trueness problems associated with the rapid immunochemical methods have been reviewed. Special attention is focused on discussion of cross-reactivity in the ELISA tests, because this rapid test dominates the routine screening practice. However, the cross-reactions reported in lateral flow test strips, fluorescence polarisation immunoassay, or immunosensors have also been addressed. The cross-reactivity of antibodies employed within immunochemistry-based analytical methods may lead overestimation of the results. Under certain conditions, specifically when controlling mycotoxin maximum limits serious problems can be encountered. Not only the structurally related mycotoxins, such as their masked (conjugated) forms, but also the unidentified matrix components are responsible for concentration overestimation of respective target analytes. The cross-reactivity phenomenon may also pose a risk of miss-interpretation of the proficiency tests results, when the assigned value becomes influenced by over-estimated results reported by users of immunochemical tests. In this paper, the current state of the knowledge on trueness problems associated with the rapid immunochemical methods have been reviewed. Special attention is focused on discussion of cross-reactivity in the ELISA tests, because this rapid test dominates the routine screening practice. However, the cross-reactions reported in lateral flow test strips, fluorescence polarisation immunoassay, or immunosensors have also been addressed. (en)
Title	Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge (en)
skos:prefLabel	Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge (en)
skos:notation	RIV/60461373:22330/14:43898063!RIV15-MZE-22330___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QI111B044), P(QI111B154)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Zachariášová, Milena Hajšlová, Jana Cuhra, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	9258
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/14:43898063
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	lateral flow device; fluorescence polarisation immunoassay; immunosensors; enzyme-linked immunosorbent assay; Proficiency testing (en)
http://linked.open.../riv/klicoveSlovo	enzyme-linked immunosorbent assay Proficiency testing fluorescence polarisation immunoassay immunosensors lateral flow device
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[99A6B42D822E]
http://linked.open...i/riv/nazevZdroje	WORLD MYCOTOXIN JOURNAL
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Comprehensive strategy for decreasing a negative impact of Fusarium spp. toxicogenic fungi infection in cereals and their derivated products. Safety of cereals bioproducts in light of occurence of Alternaria and Fusarium mycotoxins.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Hajšlová, Jana Zachariášová, Milena Cuhra, Petr
http://linked.open...ain/vavai/riv/wos	000343071600006
issn	1875-0710
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.3920/WMJ2014.1701
http://localhost/t...ganizacniJednotka	22330

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