About: Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis

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About: Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis Goto Sponge NotDistinct Permalink

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://ac.els-cdn.com/S0167701214000207/1-s2.0-S0167701214000207-main.pdf?_tid=0fe3df92-a861-11e4-8f66-00000aab0f6c&acdnat=1422609736_0d9b8721de703da1123ae5ee6262e32d
Description	Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species. Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species. (en)
Title	Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis (en)
skos:prefLabel	Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis (en)
skos:notation	RIV/60461373:22330/14:43897784!RIV15-GA0-22330___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(GAP503/10/0664), S
http://linked.open...iv/cisloPeriodika	March
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hochel, Igor Rohlová, Eva Šantrůček, Jiří Krásný, Lukáš Hynek, Radovan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	11500
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/14:43897784
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Trypsin; Mass spectrometry; MALDI-TOF; Cronobacter; Biotyper (en)
http://linked.open.../riv/klicoveSlovo	Biotyper Cronobacter MALDI-TOF Mass spectrometry Trypsin
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[584F7B2B94F2]
http://linked.open...i/riv/nazevZdroje	Journal of Microbiological Methods
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Isolation, typing and the development of immunochemical and instrumetal methods for detection and characterization of Cronobacter sp.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	98
http://linked.open...iv/tvurceVysledku	Hynek, Radovan Šantrůček, Jiří Krásný, Lukáš Růžičková, Helena Rohlová, Eva Hochel, Igor
http://linked.open...ain/vavai/riv/wos	000333852700019
issn	0167-7012
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.mimet.2014.01.008
http://localhost/t...ganizacniJednotka	22330

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