About: Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules

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About: Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0042682213006193
Description	The intracellular transport of Mason-Pfizer monkey virus (M-PMV) assembled capsids from the pericentriolar region to the plasma membrane (PM) requires trafficking of envelope glycoprotein (Env) to the assembly site via the recycling endosome. However, it is unclear if Env-containing vesicles play a direct role in trafficking capsids to the PM. Using live cell microscopy, we demonstrate, for the first time, anterograde co-transport of Gag and Env. Nocodazole disruption of microtubules had differential effects on Gag and Env trafficking, with pulse-chase assays showing a delayed release of Env-deficient virions. Particle tracking demonstrated an initial loss of linear movement of GFP-tagged capsids and mCherry-tagged Env, followed by renewed movement of Gag but not Env at 4 h post-treatment. Thus, while delayed capsid trafficking can occur in the absence of microtubules, efficient anterograde transport of capsids appears to be mediated by microtubule-associated Env-containing vesicles. The intracellular transport of Mason-Pfizer monkey virus (M-PMV) assembled capsids from the pericentriolar region to the plasma membrane (PM) requires trafficking of envelope glycoprotein (Env) to the assembly site via the recycling endosome. However, it is unclear if Env-containing vesicles play a direct role in trafficking capsids to the PM. Using live cell microscopy, we demonstrate, for the first time, anterograde co-transport of Gag and Env. Nocodazole disruption of microtubules had differential effects on Gag and Env trafficking, with pulse-chase assays showing a delayed release of Env-deficient virions. Particle tracking demonstrated an initial loss of linear movement of GFP-tagged capsids and mCherry-tagged Env, followed by renewed movement of Gag but not Env at 4 h post-treatment. Thus, while delayed capsid trafficking can occur in the absence of microtubules, efficient anterograde transport of capsids appears to be mediated by microtubule-associated Env-containing vesicles. (en)
Title	Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules (en)
skos:prefLabel	Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules (en)
skos:notation	RIV/60461373:22330/14:43897526!RIV15-GA0-22330___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GAP302/12/1895), P(LH12011)
http://linked.open...iv/cisloPeriodika	leden 2014
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Grznárová, Petra Ruml, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	11641
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/14:43897526
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Live cell-imaging; Cytoskeleton; Anterograde transport; Gag; Envelope; M-PMV (en)
http://linked.open.../riv/klicoveSlovo	Anterograde transport Live cell-imaging Cytoskeleton M-PMV Gag Envelope
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[E11D2C937C15]
http://linked.open...i/riv/nazevZdroje	Virology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of retroviral matrix protein in virus particle targeting and interaction with plasma membrane. Structure of retroviral particle and intracellular transport of its components.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	449
http://linked.open...iv/tvurceVysledku	Ruml, Tomáš Hunter, Eric Grznárová, Petra Clark, Jasmine Spearman, Paul LaCasse, Rachel Pereira, Lara E. Wen, Xiaoyun
http://linked.open...ain/vavai/riv/wos	000330094100013
issn	0042-6822
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.virol.2013.11.006
http://localhost/t...ganizacniJednotka	22330

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